Journal of South China University of Technology (Natural Science Edition) ›› 2007, Vol. 35 ›› Issue (11): 120-124.

• Biotechnology • Previous Articles     Next Articles

Purification of Glycosaminoglycan Extracted from Octopus dollfusi and Effect on Proliferation of Mouse Splenocytes

Lei Xiao-ling1  Zhao Shu-jin1  Wu Hong-mian2  Peng Xiao-na2  Yang Zhi-juan2   

  1. 1. School of Biological Science and Engineering, South China Univ. of Tech , Guangzhou 510640 ， Guangdong , China;2. College of Food Science and Tech. , Guangdong Ocean Univ. , Zhanjiang 524025 ， Guangdong , China
  • Received:2006-09-19 Online:2007-11-25 Published:2007-11-25
  • Contact: 赵树进，教授， E-mail: gzzsjzhs @ pub. guang-zhou.gd.cn E-mail:leixl@gdou.edu.cn
  • About author:雷晓凌(1963-) ，女，在职博士生，广东海洋大学教授，主要从事海洋生物活性物质的研究.
  • Supported by:

    广东省科技厅社会攻关项目(2003 C32805)

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Abstract:

In this paper , the crude glycosaminoglycan extracted from Oc.ωp旧dollfusi (COG) was refined to obtain POG, and was then purified by means of Anion-Exchange and Gel Filtration Chromatography. Mterwards , chemistrγassay and IR spectrography were adopted to determine the main compositions and structures of the products , and MIT assay was used to measure the splenocyte proliferation. The results show that the total sugar contents of POGⅠ, POG Ⅱ and POGⅠ -1 purified from the chromatography are all in the range of 26.3% -42.2% , and that there is more than 30% of total glycosaminoglycan and approximately 20% of glucuronic acid. Moreover , the IR spectrum reveals that there are typical glycosaminoglycan structure characteristics , and that COG, POG, POGⅠ, POGⅡ, POG Ⅰ -1 all significantly promote the proliferation of mouse splenocytes, especial1y POG Ⅰ -1 that has the greatest effect. It thus concluded that the glycosaminoglycans from Octopus dollfusi consisting of hexosamine and glucuronic acid have the function of immunity enhancement.

Key words: Octop&omega, dollfusi, glycosaminoglycan, purification, mouse, splenocyte, proliferation