Journal of South China University of Technology (Natural Science Edition) ›› 2015, Vol. 43 ›› Issue (11): 8-15.doi: 10.3969/j.issn.1000-565X.2015.11.002

• Food Science & Technology • Previous Articles     Next Articles

Separation，Identification and Antioxidant Activity of Glycoproteins from Millettia Speciosa Champ．

Zhao Qiang-zhong Feng Meng-ying Lin Lian-zhu Zhao Mou-ming   

  1. School of Light Industry and Food Sciences，South China University of Technology，Guangzhou 510640，Guangdong，China
  • Received:2015-04-10 Revised:2015-06-24 Online:2015-11-25 Published:2015-10-01
  • Contact: 赵谋明(1964-)，男，博士，教授，博士生导师，主要从事食品生物技术研究． E-mail:femmzhao@scut.edu.cn
  • About author:赵强忠(1976-)，男，博士，教授，博士生导师，主要从事食品生物技术研究． E-mail: qzzhao@ scut． edu． cn
  • Supported by:
    Supported by the Production，Education and Ｒesearch Cooperative Project of Guangdong Province and the Ministry
    of Education(2012B090600025)

PDF

199

Abstract: In order to understand well the structural characteristics，physical and chemical properties and biologi-cal activities of glycoprotein from Millettia Speciosa Champ． (MSCG)，MSCG was purified by means of ultrafiltra-tion，chromatography Sephadex G-75 and ODS according to its molecular mass distribution，and thus two purified fractions MSCG-1 and MSCG-2 were obtained． Then，the structures of the two fractions were identified by means of HPLC，GC，GC-MS and FT-IＲ． The results show that (1) the molecular masses of MSCG-1 and MSCG-2 are 62. 00 ku and 1. 94 ku respectively; (2) MSCG-1 contains 50. 22% protein and 38. 07% sugar，and it is mainly composed of → 4，6)-Galp-(1 → and Araf-(1 → ; (3) MSCG-2 contains 69. 37% protein and 6. 70% sugar，and its main chain is composed of → 6)-Glcp-(1 → and has a high branch degree; (4) MSCG-1 and MSCG-2 both con-tain O-link bonds; (5) for the amino acid which may be of antioxidant activities，its content in MSCG-2 is 31. 3% higher than that in MSCG-1; (6) the oxygen radical absorbance capacities (ORAC) of MSCG-1 and MSCG-2 are (56. 10 ±18. 49) and (1077. 19 ± 60. 82) μmol/g; and (7) the antioxidant activity of MSCG-2 is significantly higher than those of MSCG and MSCG-1，which means that the molecular composition，molecular mass and struc-tural characteristics of glycoprotein determine their antioxidant activities．

Key words: Millettia Speciosa Champ．, glycoprotein, separation, purification, structure identification, antioxidants

CLC Number: