Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (12): 145-150.doi: 10.3969/j.issn.1000-565X.2010.12.028

• Biological Engineering • Previous Articles    

Purification and Structure Identification of Phytohemagglutinin-L4

Zhang Xiao-yuan1  Guo Yong2  Wu Hui 3 Zhao Qi4  Huang Min2   

  1. 1.Industrial Technology Research Institute,South China University of Technology,Guangzhou 510640,Guangdong,China;2.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;3.School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China;4.Guangzhou Hezhu Bio-Tech Co.,Ltd.,Guangzhou 510633,Guangdong,China
  • Received:2010-01-04 Revised:2010-06-01 Online:2010-12-25 Published:2010-12-25
  • Contact: 张晓元（1964-），男，博士，副教授，主要从事生物医药、天然产物研究． E-mail:xyzh@scut．edu．cn
  • About author:张晓元（1964-），男，博士，副教授，主要从事生物医药、天然产物研究．
  • Supported by:

    中国博士后基金资助项目（2004036497）; 广东省科技计划项目（2005B33001008）

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Abstract:

Mitogenetic glycoprotein PHA-L4 with high activity was obtained from phytohemagglutinin（PHA） after the separation and purification by means of anion exchange chromatography and biological activity inspection.The structure of the product was then identified by means of SDS-PAGE,mass spectrometry,N-terminal sequencing measurement and peptide mass fingerprint.The results indicate that PHA-L4 is a kind of glycoprotein tetramer composed of 4 PHA-L subunits,with a relative molecular mass of 120000,and that each subunit is heterogeneously glycosylated at the 12th Asn to a certain degree.

Key words: phytohemagglutinin, purification, identification, mass spectrometry, N-terminal sequencing, peptide mass fingerprint