Journal of South China University of Technology (Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (11): 143-148.doi: 10.3969/j.issn.1000-565X.2014.11.021

• Biological Engineering • Previous Articles    

Directional Osteogenic Differentiation of hMSCs and its Biocompatibility with PLGA/TCP

Wang Fei1 Wang Yan2 Cheng Lü- sha2 Xie Mu- yuan3 Zhang Li- jun2 Liang Shi- zhong1   

  1. 1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China; 2.School of Applied Chemistry and Biotechnology,Shenzhen Polytechnic,Shenzhen 518055,Guangdong,China; 3.College of Life Science and Biopharmaceutical,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China
  • Received:2014-01-10 Revised:2014-02-24 Online:2014-11-25 Published:2014-11-17
  • Contact: 张丽君(1975-)，女，博士，副教授，主要从事细胞与分子生物学研究．梁世中(1943-)，男，教授，主要从事发酵与生化工程研究． E-mail:c7zlj@szpt.edu.cn|fesliang@scut.edu.cn
  • About author:王飞(1977-)，男，博士生，主要从事细胞培养和发酵工程研究．E-mail:freefray@163．com
  • Supported by:

    广东省教育部产学研结合项目(2012B091100408)

PDF

252

Abstract:

In order to verify the directional osteogenic differentiation of human mesenchymal stem cells (hMSCs),the corresponding experiments were conducted with the hMSCs cultured in the osteogenic differentiation medium as the osteogenic differentiation group and those in the complete medium as a control group.Von Kassa experiment shows that hMSCs has a good osteogenic differentiation ability with obvious calcium nodules.Alkaline phosphatase (ALP) test demonstrates that the ALP activity of the osteogenic differentiation group is higher than that of the con- trol group (P ＜0.01),and reaches a peak in twelve days and then enters into a plateau phase.In order to verify the biocompatibility of hMSCs with poly lactide- co- glycolide (PLGA) /TCP,the corresponding analyses are made with the hMSCs cultured in PLGA/TCP as the 3D culture group and those on plate as the 2D control group.SEM results show that adhesion,proliferation and osteogenic differentiation of hMSCs occur in PLGA/TCP.MTT test in- dicates that the cell proliferation rate of the 3D culture group in five and seven days (P ＜0.01) is obviously higher than those of the 2D control group.ALP test also demonstrates that the osteogenic differentiation of the 3D culture group in three,five and seven days (P ＜0.05) are higher than those of the 2D control group,which indicates that PLGA/TCP possesses good cellular biocompatibility with hMSCs and they have a potential to produce tissue engi- neering bones.

Key words: bone tissue engineering, human mesenchymal stem cells, poly lactide- co- glycolide, tricalcium phos- phate, cell proliferation, osteogenic differentiation, biocompatibility