Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (5): 132-137.doi: 10.3969/j.issn.1000-565X.2011.05.023

• Biological Engineering • Previous Articles     Next Articles

Isolation and Identification of Violet-Blue Pigment from Streptomyces vietnamensis

Deng Ming-rong1,2  Zhu Chun-hua2  Guo Jun2  Zhu Hong-hui2   

  1. 1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;2.Guangdong Provincial Key Laboratory of Microbial Culture Collection andApplication,Guangdong Institute of Microbiology,Guangzhou 510070,Guangdong,China
  • Received:2010-10-12 Revised:2011-02-14 Online:2011-05-25 Published:2011-04-01
  • Contact: 朱红惠(1970-)，女，研究员，主要从事微生物资源与利用、微生物与植物互作等研究 E-mail:zhuhonghui66@yahoo.com.cn
  • About author:邓名荣(1979-)，男，博士生，主要从事微生物资源与利用、生物活性物质研究
  • Supported by:

    广东省科技计划项目(2008B021400005)；广州市科技计划项目(2008J1-C091)；广东省科学院科技创新引导项目(cx200702)

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Abstract:

In order to develop natural pigments from microbes as food colorants,the production level of Streptomyces vietnamensis for violet-blue pigment was tested in different media,and such four parameters as the inoculation quantity,the culture temperature,the rotation speed and the fermentation time were orthogonally optimized.Then,the constituents of the pigment were isolated and purified by means of the silica gel column chromatography,the sephadex gel filtration chromatography,the thin-layer chromatography and the high-performance liquid chromatography,and the structure of the pigment was identified by means of the nuclear magnetic resonance and the mass spectrometry.The results show that Gauze＇s synthetic No.1 medium is suitable for the industrial production of violet-blue pigment due to its highest productivity,and that the optimal fermentation conditions are as follows： an inocu-lation quantity of 5%,a culture temperature of 30 ℃,a rotation speed of 180 r/min and a culture time of 7 d.Moreover,seven compounds,namely granaticin,granaticin B,MM44785,L-rhodinoside-deglycosylated MM44785 as well as quinizarin-derived pigments Zg,Zgg and dihydro-Zg,can be isolated and purified from the crude extract,among which dihydro-Zg and L-rhodinoside-deglycosylated MM44785 both possess novel structures.

Key words: Streptomyces vietnamensis, natural pigment, purification, identification, structure, granaticin