Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (5): 107-114.

• Biological Engineering • Previous Articles     Next Articles

Isolation and Purification of Novel Fibrinolytic Enzymes Obtained from Fermentation of Cordyceps militaris

Liu Xiao-lan  Zhang Wen-shu  Zheng Xi-qun  Shen Yuan  Sun Ying   

  1. The Ordinary University Key Laboratory of Agricultural Products Processing,Qiqihar University,Qiqihar 161006,Heilongjiang,China
  • Received:2011-12-05 Revised:2012-02-27 Online:2012-05-25 Published:2012-03-31
  • Contact: 刘晓兰(1962-) ，女，博士，教授，主要从事发酵工程与应用酶学研究． E-mail:liuxiaolan001@126.com
  • About author:刘晓兰(1962-) ，女，博士，教授，主要从事发酵工程与应用酶学研究．
  • Supported by:

    国家自然科学基金资助项目( 31171744) ; 齐齐哈尔大学创新科研项目( YJSCX2010-009X)

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Abstract:

In their earlier research,the authors have found that at least two kinds of fibrinolytic enzymes can be obtained from the submerged culture of Cordyceps militaris. As a further research,this paper deals with the isolation and purification of these fibrinolytic enzymes by means of ammonium sulfate precipitation,Sephadex G-25 gel filtration chromatography,Phenyl-Sepharose HP hydrophobic interaction chromatography,CM-Sepharose FF weak-cation exchange chromatography and Superdex 75 gel filtration chromatography. After the purification,two kinds of fibrinolytic enzymes are obtained,namely fibrinolytic enzyme I with a specific activity of 1467.44U/mg,a purification fold of 36.07 and a recovery of 5.79%,as well as fibrinolytic enzyme II with a specific activity of 1681.58U/mg,a purification fold of 41.33 and a recovery of 4.00%. SDS-PAGE results indicate that the two kinds of fibrinolytic enzymes,whose relative molecular mass are respectively about 28000 and 32000,are both electrophoretic pure.

Key words: Cordyceps militaris, submerged culture, fibrinolytic enzyme, purification, chromatographic separation