Preparation of Chloramphenicol Molecularly Imprinted Solid-phase Extraction Cartridge and the Optimization of Extraction Conditions

Journal of South China University of Technology(Natural Science Edition) ›› 2004, Vol. 32 ›› Issue (7): 51-55.

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Preparation of Chloramphenicol Molecularly Imprinted Solid-phase Extraction Cartridge and the Optimization of Extraction Conditions

Chen Xiao-xia¹ Yue Zhen-feng² Zheng Wei-ping² Xie Li-qi² Liang Shi-zhong¹

1．College of Food and Biological EngineeringSouth China Univ．of Tech．Guangzhou510640GuangdongChina；
2．Inspection Center of Import ＆Export FoodShenzhen Entry-Exit Inspection＆Quarantine Bureau of the Peopleʾs Republic of ChinaShenzhen518067GuangdongChina

Received:2004-01-11 Online:2004-07-20 Published:2015-09-09
Contact: 陈小霞（1975－）女博士生主要从事生物制药的研究． E-mail:xiaoxia-ch＠163.com
About author:陈小霞（1975－）女博士生主要从事生物制药的研究．

Abstract

Abstract: The molecularly imprinted solid-phase extraction （SPE） cartridge of chloramphenicol（CAP） was prepared by adopting molecular imprinting techniqueand its absorption ability as well as desorption conditions was investigatedon the basis of which the working conditions of SPE cartridgeincluding the solvent for CAPthe rinse and elution con-ditionswere ascertained．The results indicate thatcompared to the traditional C18cartridgesthe molecularly imprinted solid-phase extraction cartidge is of great advantagesthat isit can use methano- l water solution with high methanol content as the rinse solutionacetonitrile-water solution with40％（volume fraction） acetonitrile as the elution solutionand it is of a greater cartridge capacity than C18cartridges．All these advantages make it possible to apply the SPE car-tridge to the CAP detection of actual bio-productsthus resolving the problems existing in the CAP residue detection at present．

Key words: molecular imprinting technique, solid-phase extraction, chloramphenicol, drug residue, detection

Chen Xiao-xia Yue Zhen-feng Zheng Wei-ping Xie Li-qi Liang Shi-zhong. Preparation of Chloramphenicol Molecularly Imprinted Solid-phase Extraction Cartridge and the Optimization of Extraction Conditions[J]. Journal of South China University of Technology(Natural Science Edition), 2004, 32(7): 51-55.

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Preparation of Chloramphenicol Molecularly Imprinted Solid-phase Extraction Cartridge and the Optimization of Extraction Conditions

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