Journal of South China University of Technology(Natural Science Edition) ›› 2022, Vol. 50 ›› Issue (4): 119-128.doi: 10.12141/j.issn.1000-565X.210383

Special Issue: 2022年化学化工

• Chemistry & Chemical Engineering • Previous Articles     Next Articles

Purification of Kapok Flavonoids by Macroporous Resin and Its Anti-Acne Efficacy

ZHENG Bisheng1 WEI Ruijing2 GUO Chaowan3 ZHOU Lin4 WANG Juan5 HU Lu6 WANG Lihua7   

  1. 1． School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China;
    2． Guangdong Marumi Biotechnology Co． ，Ltd． ，Guangzhou 510530，Guangdong，China;
    3． Guangdong Pharmaceutical University，Guangzhou 510006，Guangdong，China
  • Received:2021-06-11 Revised:2021-08-31 Online:2022-04-25 Published:2021-09-10
  • Contact: 郑必胜 (1966-)，男，博士，副教授，主要从事天然活性成分制备及其在化妆品中的应用研究 E-mail:fe-bzheng@scut.edu.cn
  • About author:郑必胜 (1966-)，男，博士，副教授，主要从事天然活性成分制备及其在化妆品中的应用研究

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Abstract: Study the extraction process of kapok flavonoids and evaluate its anti-acne efficacy. Using Guangdong Zhaoqing kapok as raw material, after ultrasonic-assisted alcohol extraction, the most suitable macroporous resin type was investigated and the purification process of the crude kapok flavonoids was determined. The in vitro inhibition zone experiment method was used to determine the purified and refined kapok flavonoids. The inhibitory effect of Staphylococcus epidermidis and Propionibacterium acnes; the in vitro whitening activity method was used to determine the inhibition rate of kapok flavonoids on tyrosinase, and the whitening activity of kapok flavonoids was further verified by the zebrafish embryo melanin inhibition test; Finally, the in vitro antioxidant activity determination method was used to investigate the scavenging ability of kapok flavonoids on free radicals such as ABTS?+ and DPPH. By comparing the adsorption rate and resolution rate of 8 kinds of macroporous resins to kapok flavonoids, D101 was screened out as the best resin type, and the best static adsorption desorption conditions were investigated as follows: adsorption time 6 h,resolution time 4 h, the ethanol concentration of the analytical solution is 60%; the best dynamic adsorption analysis conditions are: loading volume 2 BV, analytical solution elution volume 0.625 BV. After purification, the purity of kapok flavonoids increased from 32.5% to 98.2%. The IC50 values of the purified kapok flavonoids on ABTS?+ and DPPH free radical scavenging rates were 0.62±0.011 mg/mL and 11.8±0.624 μg/mL, respectively.The IC50 of the inhibition rate of Kapok flavonoids on tyrosinase is 1.69±0.002 mg/mL. When the concentration of kapok flavonoids was 1.0 mg/mL, the inhibition rate of melanin in zebrafish embryos was 12%, and it had significant whitening activity.Through the inhibition zone test, when the concentration of kapok flavonoids is 12.5 mg/mL, it is moderately sensitive to Propionibacterium acnes and highly sensitive to Staphylococcus epidermidis, and this antibacterial effect presents a dose-dependent effect.In summary, the kapok flavonoids purified and prepared in this study have multiple skin care activities such as whitening, antioxidation, antibacterial and anti-acne, and have good application and development value for anti-acne products.

Key words: Kapok, Flavonoids, Extraction, Purification, Antioxidant, Whitening, Antibacterial

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