Study on CMC/CS/Ca2+ Composite Hydrogel for Transdermal Delivery of SPE in vitro and Its Antioxidant Activity

doi:10.12141/j.issn.1000-565X.220388

Abstract

Abstract:

Peptides, such as soybean peptide (SPE), play an important part in drugs targeted therapy and diagnosis. However, their intolerance for the extremely low pH and ionic barriers of the stomach makes them hard to be delivered orally. Transdermal delivery system can deliver drugs to the blood avoiding the obstacles of the gastric. In this paper, carboxymethyl cellulose/cationic starch/Ca²⁺ (CMC/CS/Ca²⁺) composite hydrogel was successfully prepared by freeze-thaw cycle, and it can overcome the barrier of penetration of water-soluble SPE in the stratum corneum during transdermal delivery. The results of particle charge detector (PCD), Fourier transform infrared spectroscopy (FT-IR) and rheology show that chemical and physical cross-linking occurs among CMC, CS and Ca²⁺, forming a stable hydrogel. Moreover, the interaction among SPE, CS and Ca²⁺ makes its mechanical properties meet the requirements of transdermal delivery. The results of scanning electron microscope (SEM) and specific surface area measurement (BET) illustrate that there is a porous 3D network structure in those composite hydrogels. The moisture content of the composite hydrogel is higher than 95.63% and remains above 97.34% after 25 min at 25 ℃. All composite hydrogels swell rapidly within 120 min, and the equilibrium swelling fraction can reach up to 636.23% at 720 min. Moreover, it is also indicated that the composite hydrogels have high affinity for water, which is the fundamental reason for improving the transdermal delivery efficiency of SPE. The results of in vitro release show that the release type of SPE releasing from hydrogel belongs to swelling controlled release type, and the maximum scavenging rates of DPPH radical and ABTS radical are 47.07% and 72.16%, respectively. The cumulative permeation per unit area of SPE is 0.55, 0.70 and 0.60 mg/cm², separately, and the cumulative penetration percentage is 84.13%, 92.93% and 92.57%, respectively. The appearance and pH value, thermostability and cold endurance of the hydrogel all meet the standards of QB/T 2872—2017. Therefore, these composite hydrogels are expected to be applied not only in transdermal delivery of peptides and protein drugs, but also in skin antioxidant care.

Key words: carboxymethyl cellulose, cation starch, soybean peptide, transdermal delivery, antioxidant activity

CLC Number:

O636.1⁺1

YE Jun, LIU Luying, HUANG Zhuosheng, et al. Study on CMC/CS/Ca²⁺ Composite Hydrogel for Transdermal Delivery of SPE in vitro and Its Antioxidant Activity[J]. Journal of South China University of Technology(Natural Science Edition), 2023, 51(1): 51-60.

Figures/Tables 13

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样品	各成分用量/g
样品	CMC	CS	Ca²⁺	SPE
CC1	0.80	1.20	0.04	0.00
CC2	0.80	1.28	0.04	0.00
CC3	0.80	1.44	0.04	0.00
CCL1	0.80	1.20	0.04	0.40
CCL2	0.80	1.28	0.04	0.40
CCL3	0.80	1.44	0.04	0.40
CCL1-3	0.80	1.20	0.24	0.40
CCL1-4	0.80	1.20	0.32	0.40
CCL1-6	0.80	1.20	0.48	0.40

来源	最大DPPH自由基清除率/%	最大ABTS自由基清除率/%
文献［19］结果	44.24	13.48
文献［36］结果	45.78	—
文献［37］结果	67.41	—
文献［38］结果	36.58	57.58
文中结果	47.07	72.16

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Study on CMC/CS/Ca²⁺ Composite Hydrogel for Transdermal Delivery of SPE in vitro and Its Antioxidant Activity

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