Journal of South China University of Technology (Natural Science Edition) ›› 2022, Vol. 50 ›› Issue (1): 23-29.doi: 10.12141/j.issn.1000-565X.210170

Special Issue: 2022年食品科学与技术

• Food Science & Technology • Previous Articles     Next Articles

Protective Mechanism of Yeast Hydrolysates Against the Oxidative Injury on Human Umbilical Vein Endothelial Cells

HUANG Yanbo1,2 SUN Weizheng1 HOU Yi2 SUN Jiangwen3 LIU Guangyi4 HU Songqing1   

  1. 1.School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China；2.State Key Laboratory of Pulp and Paper Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China；3.Hospital of South China University of Technology，Guangzhou 510640，China；4.China-Singapore International Joint Research Institute，Guangzhou 510555，Guangdong，China
  • Received:2021-03-27 Revised:2021-05-29 Online:2022-01-25 Published:2022-01-03
  • Contact: 胡松青（1972-），男，教授，博士生导师，主要从事食品蛋白质结构与功能及工业用酶制剂开发应用等方面研究。 E-mail:fesqhu@scut.edu.cn
  • About author:黄滟波（1984-），女，博士，实验师，主要从事功能食品研究。E-mail：feybhuang@scut.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China （32072157） and the Science and Technology Planning Project of Guangdong Province（2020A1414010130）

PDF

122

Abstract: To investigate the protective mechanism of yeast hydrolysate （YH） on human umbilical vein endothelial cell injury induced by H2O2，antioxidant activity of YH was studied.The results show that the median effective concentration （EC50） of YH on DPPH scavenging is 1.85 mg/mL，showing good antioxidant activity.And YH of high-dose （500 μg/mL） can significantly alleviate the cell proliferation inhibition induced by H2O2，reduce malondialdehyde （MDA） production and increase the level of superoxide dismutase （SOD），thereby resisting cell oxidative damage of HUVEC caused by H2O2.Moreover，transcriptomics sequencing reveals that YH  can significantly up-regulate the expression of genes SOD2 and HMOX1.The research results not only provide basic data for YH to reduce endothelial cell oxidative damage，but also provide a theoretical basis for the application of YH in the fields of antioxidation functional foods and medicine.

Key words: hydrolysis, peptides, antioxidant activity, endothelial cells

CLC Number: