Journal of South China University of Technology (Natural Science Edition) ›› 2017, Vol. 45 ›› Issue (9): 53-59.doi: 10.3969/j.issn.1000-565X.2017.09.008

• Food Science & Technology • Previous Articles     Next Articles

A Hydrolyzing Method of Neutral Sugar in Sugar Beet Pectin and Their Quantitative Analysis

YU Shu-juan1,2 WANG Zhi-ming1 GUO Xiao-ming1,2  LI Tai-hong1 AI Chao1 GUO Xiao-bing1   

  1. 1.School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China; 2.Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2017-02-20 Revised:2017-06-29 Online:2017-09-25 Published:2017-08-30
  • Contact: 于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究． E-mail:lfshjy@scut.edu.cn
  • About author:于淑娟( 1955-) ，女，教授，博士生导师，主要从事制糖工程、碳水化合物分子修饰研究．
  • Supported by:
    Supported by the National Natural Science Foundation of China ( 31601423) ， the Science and Technology Planning Project of Guangdong Province ( 2013B051000015) ， the Achievements Transformation Fund Project ( 2016AB009) and China Postdoctoral Science Foundation( 2016M590789)

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Abstract: In order to determine the variety and contents of the neutral sugar in sugar beet pectin ( SBP) ,a twostep hydrolysis method was proposed for the SBP.In the method,the neutral sugar in the SBP were sequentially hydrolyzed by using pectinase and trifluoroacetic acid ( TFA) .First,the SBP was degraded into the fractions of smaller relative molecular mass by pectinase.Second,the neutral sugar attached to the SBP were liberated into solution by TFA.Among three commercial pectinases,Rapidase C80 was selected as the objective pectinase because of its lower endogenous monosaccharide content and better hydrolyzing activity.It is found that the optimal hydrolysis parameters of C80 are 50℃,pH 5 and 15 h.Moreover,the effects of the TFA concentration,the temperature and the hydrolysis duration on the hydrolysis of the neutral sugar were further investigated.By compromising the hydrolysis rate and the degradation rate of the neutral sugar,suitable TFA concentration,temperature and hydrolysis duration were respectively determined to be 1mol /L,110℃ and 4 h.The HPAEC analysis results show that the proposed method has a good effect in achieving rhamnose through hydrolysis,and it exhibits a good stability and a high repeatability,which means that this method can be used to quantitatively analyze the neutral sugar in the SBP.

Key words: sugar beet pectin, neutral sugar, two-step hydrolysis, ion chromatography

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