Journal of South China University of Technology (Natural Science Edition) ›› 2019, Vol. 47 ›› Issue (3): 101-108,152.doi: 10.12141/j.issn.1000-565X.180234

• Food Science & Technology • Previous Articles     Next Articles

Antioxidant and Antitumor Activities of Myricetin In Vitro

 SU Jianyu1 HU Han1 WU Ping1 MENG Xiaofeng1 XU Zhenbo 1 ZHENG Huade 2，3   

  1.  1． School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China; 2． School of Materials Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong， China; 3． South China Institute of Collaborative Innovation，Dongguan 523000，Guangdong，China
  • Received:2018-05-17 Revised:2018-10-02 Online:2019-03-25 Published:2019-01-31
  • Contact: 郑华德( 1978-) ，男，博士，副研究员，主要从事医用生物材料研究 E-mail:hdzheng@scut.edu.cn
  • About author:苏健裕( 1979-) ，男，博士，副研究员，主要从事食品生物技术研究
  • Supported by:
    Supported by the Technology Planning Project of Guangdong Province( 2016A040402032，2016A010105020， 2016A040402016) and the Yang Fan Innovative ＆ Entrepreneurial Ｒesearch Team Project( 201312H05)

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Abstract: Antioxidant assay and ESEM observation were carried out to investigate the antioxidant and anti-bladder cancer activities of myricetin． The results show that myricetin can effectively inhibit the AAPH-induced hemolysis of erythrocytes in a dose-dependent manner． The hemolysis inhibition rate of myricetin increases to ( 93. 67 ± 0. 01) % at a concentration of 80 μmol /L． A further experiment confirms that SOD and GPx antioxidant enzyme activities are enhanced to control ＲOS and MDA generation when erythrocytes are preconditioned by myricetin． Moreover，MTT tests indicate that myricetin could suppress the EJ and T24 bladder cancer cells proliferation in a dose-related manner with the half-maximal inhibitory concentration ( IC50 ) of ( 39. 8 ± 1. 0) μmol /L and ( 49. 0 ± 0. 9) μmol /L，as well as a lower cytotoxicity on normal bladder cell SV-HUC-1 with the IC50 of ( 75. 9 ± 1. 8) μmol /L． In addition， myricetin exhibits higher antitumor activity in EJ cell than that in T24 cells due to higher intracellular uptake amount of myricetin in EJ cells． Further flow cytometric studies of EJ cell cycle distribution show that myricetin inhibits the proliferation of EJ cancer cells mainly through induction of apoptosis and S cycle arrest．

Key words: flavonoids, antioxidant activity, antitumor

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