Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (9): 127-131.doi: 10.3969/j.issn.1000-565X.2010.09.024

• Biological Engineering • Previous Articles     Next Articles

Chromatography Separation and Structure Identification of Enzymatically-Synthesized Naringin Palmitate

Huang Jian-rong  Li Bing  Zhang Xia  Li Lin   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2010-01-04 Revised:2010-02-12 Online:2010-09-25 Published:2010-09-25
  • Contact: 李琳（1962-），男，教授，博士生导师，主要从事糖类物质及其药物制备与生物利用研究．E—mail：felinli@scut．edu．cn. E-mail:joanjr@126．com
  • About author:黄建蓉（1975-），女，博士生，广东药学院高级工程师，主要从事糖类物质及其药物制备与生物利用研究．
  • Supported by:

    国家“十一五”科技支撑计划项目（2006BAD27B04）; 国家自然科学基金资助项目（20976061）; 广东省自然科学基金资助项目（9151063201000066）

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Abstract:

First,preparative high-pressure liquid chromatography was used to isolate and purify the naringin palmitate enzymatically synthesized by immobilized lipase Novozym 435 in the t-amyl alcohol system.Next,IR spectrum,mass spectrum and 13C NMR were used to identify the purified product,and the mechanism of regioselectivity of the reaction was discussed.Then,the feasible operation conditions were determined as follows： a C18 column（21.5mm×250.00mm,10μm） with methanol as the mobile phase at a flowrate of 20mL/min,an injection volume of 3mL with the concentration of 30mg/mL.In these conditions,the product with a purity of more than 99% was obtained.Finally,the purified product was identified as monoester of naringin and palmitic acid,and the regioselective acylation was found preferring the 6″-hydroxyl group of naringin glucose.

Key words: naringin palmitate, lipase, high-pressure liquid chromatography, structure, regioselectivity