Optimization of Culture Medium to Improve Monascus Yellow Pigment Hue via Response Surface Methodology

Journal of South China University of Technology (Natural Science Edition) ›› 2008, Vol. 36 ›› Issue (11): 91-95,100.

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Optimization of Culture Medium to Improve Monascus Yellow Pigment Hue via Response Surface Methodology

Zhou Bo Yang Ling Cui Si-ying Wang Ju-fang Liang Shi-zhong

School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China

Received:2008-01-09 Revised:2008-06-17 Online:2008-11-25 Published:2008-11-25
Contact: 梁世中，教授，博士生导师． E-mail:fesliang@scut．edu．cn
About author:周波（1978-），男，博士生，主要从事微生物发酵工程研究．E-mail：zhou．bo@mail．scut．edu．cn

Abstract

Abstract:

In order to improve the hue of Monascus yellow pigment, six factors including the glucose, cornstarch, calcium chloride, peptone, KH2PO4 and NH4NO2 were considered to optimize the culture medium of Monascus anka mutant MYM2 via the response surface methodology （RSM） after several single-factor tests. The results show that glucose, NH4NO3 and KH2PO4 all have great effect on the yellow pigment hue, the corresponding minimum concentrations respectively being 14.2, 6.7 and 5.7 g/L, that the theoretical hue of Monascus yellow pigment is 4.58 according to the fit-model equation, and that, through five replication experiments, the Monascus yellow pigment value and the average hue respectively reach 88. 14U/mL and 4. 21, thus concluding that the proposed model is reliable.

Key words: Monascus mutant, yellow pigment, hue, culture medium, response surface methodology, optimization

Zhou Bo Yang Ling Cui Si-ying Wang Ju-fang Liang Shi-zhong . Optimization of Culture Medium to Improve Monascus Yellow Pigment Hue via Response Surface Methodology[J]. Journal of South China University of Technology (Natural Science Edition), 2008, 36(11): 91-95,100.

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Optimization of Culture Medium to Improve Monascus Yellow Pigment Hue via Response Surface Methodology

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