Abstract:

In order to screen high-yield avilamycin-producing strain , a scheme of "random NTG mutation + glycine and 2-deoxy-D-glucose resistant rational screening" was proposed after investigating the metabolism of glucose and ammonium by Streptomyces viridocbromogenes 96 (SV -96). A glycine and 2-deoxy-D-glucose resistant mutant (SV-GT-596) with high yield was then obtained. It is found that SV -GT -596 strain is of good transmissibility， and that ,by using the strain , the yield of avilamycin reaches 56. 4 mg/L, which is 62. 3 % more than the initial yield by flake fermentation. Experimental results also indicate that the rational screening overcomes the blindness of random screening and improves the screening efficiency.

Key words: avilamycin, rational screening, glycine, 2-deoxy-D-glucose, Streptomyces viridocbromogenes