Journal of South China University of Technology (Natural Science Edition) ›› 2007, Vol. 35 ›› Issue (4): 105-111.

• Biology Science • Previous Articles     Next Articles

Screening of White-Rot Fungi and Degradation of Lignin and Pitch in Masson Pine by White-Rot Fungi

Xu Xiao -feng  He Bei-ha  Xu Li-li  Shi Hai-qiang  Lin Lu   

  1. State Key Laboratory of Pulp and Paper Engineering, South China Univ. of Tech. , Guangzhou 510640 , Guangdong , China
  • Received:2006-01-19 Online:2007-04-25 Published:2007-04-25
  • Contact: 徐晓峰(1970-),男,讲师,博士,主要从事生物技术在造纸中的应用研究. E-mail:xuxf@scut. edu.cn
  • About author:徐晓峰(1970-),男,讲师,博士,主要从事生物技术在造纸中的应用研究.
  • Supported by:

    广东省科技计划项目(2004B33301011 ) ;广州市科技计划项目( B2040390)

Abstract:

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi , 20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-re-action screening. One G strain with the lowest MyceVCol value was then inoculated onto the surface of Masson pine chip , and the degradation rates of lignin and pitch were measured after 3 , 5 , 7 , 9 days , respectively. The results indicate that , with the increase in inoculation time , the losing rate of wood mass and the elimination of methylene chloride extractive gradually increase , while the contents of Klason lignin and the totallignin decrease. It is also in-dicated by SEM that there exist obvious moldy grooves on the cell walls of Masson pine chip. Moreover , the results of the cross-sectional slice-up study show that hypha develops on the cell walls and the intercellular layers.

Key words: white-rot fungus, screening, lignin, pitch, degradation