Abstract:

The aim of this paper is to investigate the technique and influence factors of membrane crystallization in lysozyme solution. In the investigation , the supersaturation of lysozyme solution was achieved by removing the sol-vent from the solution via ultrafïltration , and lysozyme crystals were formed in the presence of precipitant. Then ,optical microscopy , SEM and IR were adopted to determine and analyze the obtained crystals. The results show that
the largest crystal with a size of O. 20 mm can be obtained with a NaCl content of 5% and a solution flow rate of 1000μrn/ s in the tangential flow ultrafiltration based on hydrophobic PES ultrafiltration membrane with a molecular weight cut-off of 5 ku , and that the obtained crystals possess good structure suitable for X-ray diffraction analysis.

Key words: membrane, crystallization, protein, lysozyme, X-ray diffraction