华南理工大学学报(自然科学版) ›› 2006, Vol. 34 ›› Issue (12): 35-40.

• 生物工程 • 上一篇    下一篇

腐乳后发酵阶段微生物肽酶系统的测定

杨佐毅1 李理2 梁世中1 杨晓泉2   

  1. 1. 华南理工大学 生物科学与工程学院, 广东 广州 510640; 2. 华南理工大学 食物蛋白工程研究中心, 广东 广州 510640
  • 收稿日期:2005-12-05 出版日期:2006-12-25 发布日期:2006-12-25
  • 通信作者: 杨佐毅(1976-) , 男, 博士生, 主要从事发酵工程研究. E-mail:yangzy2003@126. com
  • 作者简介:杨佐毅(1976-) , 男, 博士生, 主要从事发酵工程研究.
  • 基金资助:

    广东省科技攻关项目( 2003C20408, 2005B10401006)

Determination of Peptidase System of Microorganisms in Ripening of Chinese Sufu

Yang Zuo-yi1  Li Li2  Liang Shi-zhong1  Yang Xiao-quan2   

  1. 1.School of Biological Science and Enginering,South China Univ.of Tech.,Guangzhou 510640,Gnangdong,China;2.Research Center of Food Protein Ensinering,South China Univ.of Tech.,Guangzhou 510640,Gnangdong,China
  • Received:2005-12-05 Online:2006-12-25 Published:2006-12-25
  • Contact: 杨佐毅(1976-) , 男, 博士生, 主要从事发酵工程研究. E-mail:yangzy2003@126. com
  • About author:杨佐毅(1976-) , 男, 博士生, 主要从事发酵工程研究.
  • Supported by:

    广东省科技攻关项目( 2003C20408, 2005B10401006)

摘要: 采用合成底物对华南地区腐乳后发酵阶段分离得到的细菌短杆菌属(Breviba cte-rium)菌株DH1、JS3、GH4、DG6和酵母菌SCY1、JSBB2的胞内和胞外肽酶系统进行了测定. 实验结果表明, 分离得到的细菌菌株具有较高的内肽酶活力, 其亮氨酸氨肽酶、精氨酸氨肽酶、二肽酶和羧肽酶的活力很高. 而分离出的酵母菌的谷氨酸氨肽酶、赖氨酸氨肽酶活力较高, 同时具有很高的二肽酶与羧肽酶活力.

关键词: 腐乳, 发酵, 肽酶活力

Abstract:

Synthetical substrates were used to test the peptidase activities of diferent microorganisms,including Brevibacterium DH1,JS3,GH4,DG6 and Yeast SCY1,JSBB2 isolated in the ripening of sufu in South China.The intracellular an d extracellular peptidase systems of these strains were also studied.Experimental results show that the endopeptidase activities of the strains are fairly high,and that the Leu-aminopeptidaso ,Arg-aminopepti-dase,dipeptidase and carboxypeptidase activities prominently exhibit in the isolated strains.As to the two yeasts,it is of hish Glu-aminopeptidase and Lys-aminopeptidase activities as well as prolific dipeptidaso an d carboxypeptidase activities.

Key words: sufu, fermentation, peptidase activity