裙带菜多糖的结构及抗氧化、免疫调节活性

doi:10.3969/j.issn.1000-565X.2018.11.005

华南理工大学学报(自然科学版) ›› 2018, Vol. 46 ›› Issue (11): 29-38.doi: 10.3969/j.issn.1000-565X.2018.11.005

裙带菜多糖的结构及抗氧化、免疫调节活性

游丽君^1,2 黄诗铭^1,2 郑桂青^1,2 赵振刚^1,2 韩锐^1,2 孟赫诚¹

1．华南理工大学食品科学与工程学院，广东广州 510640;
2．食品营养与健康学科创新引智基地( 111 基地) ，广东广州 510640

收稿日期:2018-05-28 修回日期:2018-07-05 出版日期:2018-11-25 发布日期:2018-10-02
通信作者: 游丽君(1982) ，女，博士，副研究员，主要从事生物活性多糖、多肽结构和功能评价等的研究 E-mail:feyoulijun@scut.edu.cn
作者简介:游丽君(1982) ，女，博士，副研究员，主要从事生物活性多糖、多肽结构和功能评价等的研究
基金资助:
广东省自然科学基金资助项目( 2016A030312001) ;
广东省特支计划青年拔尖人才项目( 2015TQ01N670) ;
广州市珠江新星项目( 201610010096)

Structural，Antioxidant Activity and Immunomodulatory Activity of Polysaccharides Isolated from Undaria pinnatifida#br#

YOU Lijun^1,2 HUANG Shiming^1,2 ZHENG Guiqing^1,2 ZHAO Zhengang^1,2 HAN Rui^1,2 MENG Hecheng¹

1． School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China;
2． Food Nutrition and Human Health Overseas Expertise Introduction Center for Discipline Innovation ( 111 Center) ，Guangzhou 510640，Guangdong，China

Received:2018-05-28 Revised:2018-07-05 Online:2018-11-25 Published:2018-10-02
Contact: 游丽君(1982) ，女，博士，副研究员，主要从事生物活性多糖、多肽结构和功能评价等的研究 E-mail:feyoulijun@scut.edu.cn
About author:游丽君(1982) ，女，博士，副研究员，主要从事生物活性多糖、多肽结构和功能评价等的研究
Supported by:
The Natural Science Foundation of Guangdong Province( 2016A030312001)

摘要/Abstract

摘要： 本文采用水提醇沉法制备裙带菜多糖（UPP），提取率为11.00 ± 0.25%，UPP由葡萄糖、木糖、甘露糖、阿拉伯糖、岩藻糖、半乳糖、鼠李糖组成，分子量为691.05 kDa。体外抗氧化实验测得UPP的氧自由基吸收（ORAC）能力、ABTS自由基清除能力和DPPH自由基清除能力分别为164.54 ± 4.85 μmol TE/g，64.93 ± 4.76 μmol Trolox/g和46.80 ± 6.06 μmol Trolox/g。选用RAW264.7细胞对UPP的体外免疫调节活性进行研究，发现UPP在50~1500 μg/mL浓度范围内对该细胞无明显毒性，且可显著增强该细胞的吞噬能力，当UPP浓度为600 μg/mL时，相对细胞吞噬能力达113.22 ± 2.75%。当UPP浓度为600 μg/mL时，促RAW264.7细胞释放NO量达12.60 ± 1.01 μM；UPP在100~800 μg/mL浓度范围内显著上调iNOS mRNA的表达量。以上研究结果表明，裙带菜多糖具有良好的体外抗氧化能力和免疫调节活性作用，可应用于保健食品工业生产中。

关键词: 裙带菜, 多糖, 抗氧化活性, 免疫调节活性, 结构分析

Abstract: Undaria pinnatifida, which is mainly distributed in Liaoning, Shandong and Zhejiang province in China, is a kind of edible seaweed belongs to Phaeophyta. In the present study, a sulfated polysaccharide named as UPP was extracted by hot water from Undaria pinnatifida with a yield of 11.00 ± 0.25%. The molecular weight of UPP was 691.05 kDa, and the contents of carbonhydrate, uronic acid, sulfate and protein were 50.02 ± 1.14%, 14.98 ± 0.52%, 11.19 ± 0.40% and 2.37 ± 0.17%, respectively. UPP was composed by glucose, xylose, mannose, arabinose, fucose, galactose and rhamnose, while glucose and xylose contributed to a total molar percentage of 71.39%. UPP consisted of (1→2) or (1→4) glycosidic bond by 62.36% as well as (1→3) glycosidic bond by 35.78%. Fourier transform-infrared (FT-IR) spectra indicated that UPP have characteristic functional groups of polysaccharides. Three different methods were applied to investigate the antioxidant activity of UPP in vitro. The results showed that oxygen radical absorbance capacity(ORAC), ABTS scavenging capacity value and DPPH scavenging capacity value of UPP were 164.54 ± 4.85 μmol TE/g，64.93 ± 4.76 μmol Trolox/g and 46.80 ± 6.06 μmol Trolox/g. In addition, the immunomodulatory activity of UPP was examined by murine macrophage RAW264.7 cells model. The results indicated that UPP presented unsinificant cytotoxicity to RAW264.7 cells with the concentration of 50~1500 μg/mL. Meanwhile, RAW264.7 cells showed the strongest pinocytic capability (113.22 ± 2.75% to the control) when the concentration of UPP was 600μg/mL. Furthermore, UPP obviously promoted the production of NO, which ranked the highest when the concentration of UPP was 600μg/mL. The mRNA of iNOS increased significantly with the concentration of UPP increased from 100~800μg/mL. These results above suggested that UPP possessed excellent antioxidant ability and immunodulatory activity and could be applied to industrial production of healthy foods as an effective component.

Key words:

Undaria pinnatifida, polysaccharide, antioxidant activity, immunomodulatory activity, structural analysis

中图分类号:

TS201.2

游丽君黄诗铭郑桂青赵振刚韩锐孟赫诚. 裙带菜多糖的结构及抗氧化、免疫调节活性[J]. 华南理工大学学报(自然科学版), 2018, 46(11): 29-38.

YOU Lijun HUANG Shiming ZHENG Guiqing ZHAO Zhengang HAN Ｒui MENG Hecheng. Structural，Antioxidant Activity and Immunomodulatory Activity of Polysaccharides Isolated from Undaria pinnatifida#br#[J]. Journal of South China University of Technology(Natural Science Edition), 2018, 46(11): 29-38.

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裙带菜多糖的结构及抗氧化、免疫调节活性

Structural，Antioxidant Activity and Immunomodulatory Activity of Polysaccharides Isolated from Undaria pinnatifida#br#

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