关键词: 河蚬, 酶解物, 抗氧化活性, 乙醇, LO2 细胞

Abstract: The antioxidant activity of the Corbicula fluminea hydrolyzates obtained by using different proteases was evaluated by measuring the scavenging activity on free radical DPPH and by oxygen radical absorbance capacity ex- periments.Then，the effect of the ethanol concentrations on LO2 cells as well as the protective effect of the hydroly- zates on the cytotoxicity of LO2 cells induced by ethanol was assessed by using the methyl thiazolyl tetrazolium (MTT) assay and by determining the activities of alanine transaminase and aspartate transaminase.Finally,the a- mino acid composition of the sample,which is the most potent antioxidant,was analyzed.The results show that the hydrolyzates have certain antioxidant ability,and that,the hydrolyzates of the concentration of 300 ～400mg/L have no cytotoxic effect on the proliferation of LO2 cells,and they can increase the cell viability of the ethanol-induced LO2 cells,of which the protective effects of the pancreatin and flavourzyme hydrolyzates are significantly better than the other ones.Meanwhile,the pancreatin and flavourzyme hydrolyzates can significantly inhibit the ethanol-in- duced ALT and AST release in LO2 cells,and the contents of the antioxidant amino acids in the pancreatin and fla- vourzyme hydrolyzates are respectively 75. 69% and 75. 06%.

Key words: Corbicula fluminea, hydrolysate, antioxidant activity, ethanol, LO2 cell