硫酸酯化裂褶多糖的制备及其抗凝血活性

doi:10.12141/j.issn.1000-565X.210122

华南理工大学学报（自然科学版） ›› 2021, Vol. 49 ›› Issue (11): 9-18.doi: 10.12141/j.issn.1000-565X.210122

所属专题： 2021年食品科学与技术

硫酸酯化裂褶多糖的制备及其抗凝血活性

郑必胜¹伍磊¹ 周林² 裴运林³

1.华南理工大学食品科学与工程学院，广东广州 510640；2.广东药科大学生命科学与生物制药学院，
广东广州 511436；3.广东丸美生物科技股份有限公司，广东广州 510530

收稿日期:2021-03-10 修回日期:2021-04-30 出版日期:2021-11-25 发布日期:2021-11-01
通信作者: 郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。 E-mail:febzheng@scut.edu.cn
作者简介:郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。
基金资助:
广东省科技计划项目（2020A050503017）

Preparation and Anticoagulant Activity of Sulfated Schizophyllan Polysaccharides

ZHENG Bisheng¹ WU Lei¹ ZHOU Lin² PEI Yunlin³

1.School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China；2.School of Life Sciences and Biopharmaceuticals，Guangdong Pharmaceutical University，Guangzhou 511436，Guangdong，China；3.Guangdong Marumi Biotechnology Co.，Ltd.，Guangzhou 510530，Guangdong，China

Received:2021-03-10 Revised:2021-04-30 Online:2021-11-25 Published:2021-11-01
Contact: 郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。 E-mail:febzheng@scut.edu.cn
About author:郑必胜（1966-），男，博士，副教授，主要从事裂褶多糖的制备及改性研究。
Supported by:
Supported by the Science and Technology Program of Guangdong Province, China（2020A050503017）

摘要/Abstract

摘要： 文中主要探究裂褶多糖硫酸酯化的制备工艺及其抗凝血活性。采用单因素实验、响应面法优化三氧化硫-吡啶法制备硫酸酯化裂褶多糖（S-SPG）的工艺，并通过APTT（活化部分凝血活酶时间）、TT（凝血酶时间）、PT（凝血酶原时间）经典凝血实验探究其抗凝血功能。结果表明：当三氧化硫-吡啶复合物与裂褶多糖的质量比为4、反应温度为70℃、反应时间为3h时，S-SPG的取代度最高，为1.85。红外光谱和13C核磁数据表明裂褶多糖成功引入了硫酸基团。抗凝血实验表明，裂褶多糖经硫酸酯化后具有较强的抗凝血活性，对APTT、TT具有延长作用，对PT无影响，且其抗凝活性与浓度、取代度呈正相关。取代度最高的S-SPG3具有最强的抗凝血活性，当质量浓度为0.2g/L时，其APTT为184.35s，是同质量浓度下S-SPG2的1.86倍、S-SPG1的2.49倍，其TT超过90s。

关键词: 硫酸酯化裂褶多糖, 抗凝血活性, 三氧化硫-吡啶法, 经典凝血实验

Abstract: This paper mainly introduced the preparation and anticoagulant activity of sulfated Schizophyllan polysaccharides （S-SPG）.The single-factor and response surface method were used to optimize the preparation of polysaccharides-SPG with the sulfur trioxide-pyridine method.The anticoagulant activity was evaluated through APTT，TT，and PT assays.The results show that when the mass ratio of sulfur trioxide-pyridine complex to Schizophyllan polysaccharides （SPG） is 4，the reaction temperature is 70℃，and the reaction time is 3h，the degree of substitution of S-SPG was the highest （1.85）.The FT-IR and 13C NMR spectroscopy data proved that the sulfated group was successfully inserted into schizophyllan.Anticoagulant assays show that sulfated Schizophyllan polysaccharides exhibits strong anticoagulant activity and prolongs APTT and TT，but has no effect on PT.The anticoagulant acti-vity is positively correlated with the concentration and degree of substitution.S-SPG3 with the highest degree of substitution shows the strongest anticoagulant activity.When the mass concentration is 0.2g/L，the APTT values of S-SPG3 is 184.35s，which is 1.86 times of S-SPG2 and 2.49 times of S-SPG1 under the same concentration.Besides，the TT values of S-SPG3 was over 90 s at the mass concentration of 0.2g/L.

Key words: sulfated Schizophyllan polysaccharides, anticoagulant activity, sulfur trioxide-pyridine method, coagulation assays

中图分类号:

O629.12

郑必胜, 伍磊, 周林, 等. 硫酸酯化裂褶多糖的制备及其抗凝血活性[J]. 华南理工大学学报（自然科学版）, 2021, 49(11): 9-18.

ZHENG Bisheng, WU Lei, ZHOU Lin, et al. Preparation and Anticoagulant Activity of Sulfated Schizophyllan Polysaccharides[J]. Journal of South China University of Technology (Natural Science Edition), 2021, 49(11): 9-18.

硫酸酯化裂褶多糖的制备及其抗凝血活性

Preparation and Anticoagulant Activity of Sulfated Schizophyllan Polysaccharides

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