Journal of South China University of Technology(Natural Science Edition) ›› 2003, Vol. 31 ›› Issue (9): 70-75.

• Chemistry & Chemical Engineering • Previous Articles     Next Articles

Determination of the Antibiotic Susceptibilities of Four Marine Pathogenic Vibrios

Wang Q-i jun 1 Zhang Shu-i hua 1 Sung Chang-keun 2 Xu Bao-jun 2 Li Chang-tian 2 Baek Seoung-young 3   

  1. 1．College of Food ＆Biological Engineering‚South China Univ．of Tech．‚Guangzhou510640‚China； 2．Dept．of Food Sci．＆Tech．‚College of Agricutural Life Sci．‚Chungnan National Univ．‚Taejon305－764‚Korea； 3．Daedok Biotechnique Inc．‚Taejon305－764‚Korea
  • Online:2003-09-20 Published:2022-05-23
  • About author:Wang Q-i jun （born in1972）‚male‚Ph．D‚lecturer‚mainly researches on bioengineering and food ana-lysis．

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Abstract: A novel and sensitive thin-layer chromatography（TLC） method for specific and simultaneous detection of both lactone form Monacolin K and acid form Monacolin K is developed in this study．The TLC works in the following conditions： The TLC plate was Silica Gel60‚RP－18F254‚0．5mm‚chemically modified layer‚Merck‚ Germany；The solvent system was chloroform∶methanol ∶ammonium hydroxide＝25∶3∶1（volume ratio）；Monitoring the TLC under365nm after heating it with a slight coating of the charring reagent 30％ （volume fraction） H2SO4ethanol on the TLC plate‚allowed the specific detection of Monacolin K and the low detection limit was first time brought down to30ng．The present TLC method is suitable for the routine qualitative and sem-i quantitative analysis of the bulk materials containing Monacolin K． 

Key words: thin-layer chromatograhpy, lactone form Monacolin K, acid form Monacolin K

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