Journal of South China University of Technology (Natural Science Edition) ›› 2005, Vol. 33 ›› Issue (12): 29-32,51.

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Graded Purification and Structure Analysis of P0lysaccharides from Morinda officinalis How

He Chuan-bo  Chen Ling  Li Lin  Guo Si-yuan  Li Bin   

  1. College of Light Chemistry and Food Science,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2005-04-01 Online:2005-12-25 Published:2005-12-25
  • Contact: 何传波(1978-)，男，博士生，主要从事多糖物质及其综合利用研究 E-mail:hwctbt@sina.com
  • About author:何传波(1978-)，男，博士生，主要从事多糖物质及其综合利用研究
  • Supported by:

    广东省科技攻关项目(2003C104025)

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Abstract:

Th is paper aims at the graded purification ficinalis How.During the investigation,the Morinda and structure analysis of the polysaccharides from Morinda ofofficinalis How polysaccharides(MOHPs)were first obtained bv the extraction with hot water and subsequently by the precipitation with ethanol.Then,four polysaccharide frac-tions named respectively MOHP-Ⅰ,MOHP-Ⅱ,MOHP-Ⅲ and MOHP-Ⅳ were isolated by means of Anion-Ex-change Chromatography and Gel Filtration Chromatography.Finally,the structures of MOHP-Ⅰ and MOHP-Ⅲ were Dreliminarily analyzed by means of FT-IR,UV-IR,LPC and GPC.The results show that MOHP-Ⅰ is a polysac-charide composed of glucose and fructose,with a molecular mass of 2 ku and a typical structure with furan ring,and that MOHP-Ⅲ is a protein.bound polysaccharide composed of arabinose,xylose,glucose,fructose and galac‘
tose.with a molecular mass of 35 ku.It is also demonstrated by the results of high-perform ance Gel Perm eation Chromatography that MOHP-Ⅰand MOHP-Ⅲ can both be isolated with a high purity.

Key words: Morinda offwinalis How polysaccharide, purification, structure analysis