Journal of South China University of Technology (Natural Science Edition) ›› 2014, Vol. 42 ›› Issue (10): 44-49,56.doi: 10.3969/j.issn.1000-565X.2014.10.008

• Materials Science & Technology • Previous Articles     Next Articles

Production and Cell Compatibility of Medical Bacterial Cellulose

Li Li-feng1, 2  Ren Li1, 2  Liu Sa 1, 2  Wang Ying-jun1, 2   

  1. 1. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China; 2.National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, Guangdong, China
  • Received:2014-03-05 Revised:2014-04-14 Online:2014-10-25 Published:2014-10-01
  • Contact: 王迎军（1954-），女，教授，博士生导师，主要从事生物医用材料研究. E-mail:imwangyj@163.com
  • About author:李立风（1979-），女，博士生，主要从事医用生物材料的研究，E-mail:lf.l@foxmail.com
  • Supported by:

    国家自然科学基金资助项目（50803018，51273072）；广东省科技计划项目上（2012A080203010，2012A080800015）

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Abstract:

A medical bacterial cellulose medium with simple and definite components waswere obtained by adding trace elements with coconut juice as the basic carbon source and the growth factor source, yeast extract as the nitrogen source and ethanol as the addition carbon source. The medium contains components with relatively stable content and can improve the fermentation rate and the yield of bacterial cellulose, and the product thickness can meet various medical requirements. Then, alkali is used to purify the fermentation product by combining SDS or ultrasound or ethanol. The results show that alkali and SDS treatment procedure has the best purification effect, and that the purified sample possesses a content of impurity nitrogen below the detection limit and a high transmittance which is close to cornea in vivo in the visible region, and it exhibits a good biocompatibility  withto L929 cells.

Key words: bacterial cellulose, medium, purification, cell compatibility, biomaterials

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