Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (5): 149-153.doi: 10.3969/j.issn.1000-565X.2011.05.026

• Biological Engineering • Previous Articles     Next Articles

Screening of Oxygen-Tolerance Phenotypes in Hydrogen-Producing Strains of Chlamydomonas moewusii with Ultraviolet Radiation

Zhang Yan-ting  Fan Xiao-lei  Li Xiao-ping  Guo Rong-bo   

  1. Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,Shandong,China
  • Received:2010-05-11 Revised:2010-11-10 Online:2011-05-25 Published:2011-04-01
  • Contact: 郭荣波(1970-)，男，研究员，博士生导师，主要从事生物制氢及工业沼气研究 E-mail:guorb@qibebt.com.cn
  • About author:张彦婷(1985-)，女，博士生，主要从事微藻制氢研究
  • Supported by:

    国家自然科学基金资助项目(40806062，20877006)；山东省自然科学基金青年基金资助项目(ZR2009EQO11)；山东省科技攻关项目(2010GHY10504)；中国科学院知识创新工程重要方向项目(KGCX2-Yw-373-2)

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Abstract:

In order to reduce the sensitivity of hydrogenases in green algae to O2,four mutants of Chlamydomonas moewusii trains with improved oxygen-tolerance and hydrogen-evolving activities,namely Mu-1,Mu-2,Mu-3 and Mu-4,were generated via ultraviolet mutagenesis and were screened following an exposure to metronidazole and applied oxygen.Then,the screened strains were characterized by the hydrogenases activity in vivo,the respiration activity and the photosystem Ⅱ activity of algal cells.The results show that the hydrogenase activities in vivo of the four mutants are respectively 1.69,2.86,1.06 and 1.80 times that of the wild type,that the residual activities of the mutants after a treatment with 1% O2（volume fraction） increase respectively from the original（20.00±2.47）% to（34.40±4.88）%,（32.50±2.85）%,（55.60±3.30）% and（43.10±8.73）%,and that,there are no obvious differences in photosynthesis and respiration during the growth of Mu-1,Mu-2 and the wild type.

Key words: hydrogenase, O2-tolerance, ultraviolet-irradiation, Chlamydomonas moewusii