Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (5): 143-148.doi: 10.3969/j.issn.1000-565X.2011.05.025

• Biological Engineering • Previous Articles     Next Articles

Cloning of TOR from Thellungiella halophila and Its Role in Plant Growth

Wang Zhen  Wu Chun-xia  Hu Yu-ting  Zhang Hui  Zhao Yan-xiu   

  1. Shandong Provincial Key Laboratory of Plant Stress Research,Shandong Normal University,Jinan 250014,Shandong,China
  • Received:2010-11-18 Online:2011-05-25 Published:2011-04-01
  • Contact: 赵彦修(1954-)，男，教授，博士生导师，主要从事植物耐逆研究．E-mail:arabidopsis@sdnu．edu．cn E-mail:musicpianowz@163.com
  • About author:王臻(1983-) ，女，博士生，主要从事植物耐逆分子生物学研究．
  • Supported by:

    国家“973”计划项目( 2006CB100104) ; 国家“863”计划项目( 2007AA021402)

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Abstract:

Mammalian target of rapamycin ( TOR) plays an important role in the growth regulation and metabolism of animals and plants. In order to reveal the function of TOR in plants,TOR gene of Thellungiella halophila ( T. halophila) was cloned,and the full lengths of ThTOR cDNA and genomic DNA were obtained by polymerase chain reaction ( PCR) and rapid amplification of cDNA ends ( RACE) . Then,RNAi vector ThTOR-RNAi was constructed
and the transgenic lines were obtained by transforming ThTOR-RNAi into T, halophila. The results show that the full-length ThTOR cDNA contains 7836 base pairs and encodes a protein with 2479 amino acids,that a high degree of homology ( 97. 5%) exists between the proteins encoded by ThTOR and AtTOR,that the full-length ThTOR genomic DNA is of 19 162 base pairs,and that obvious phenotypes of slow growth as well as flowers and seeds abortion occur in T. halophila ThTOR-RNAi transgenic lines.

Key words: mammalian target of rapamycin, Thellungiella halophila, cloning, genetic transformation, RNA interference