Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (5): 144-148.doi: 10.3969/j.issn.1000-565X.2010.05.028

• Biological Engineering • Previous Articles    

Cloning and Expression of Type Ⅲ Polyketide Synthase Gene in Fallopia multiflora

Sheng Shu-jing1  Zhao Shu-jin2   

  1. 1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;2.General Hospital of Guangzhou Military Command,Guangzhou 510010,Guangdong,China
  • Received:2009-07-31 Revised:1900-01-01 Online:2010-05-25 Published:2010-05-25
  • Contact: 赵树进（1958-），男，教授，博士生导师，主要从事中药药理学研究．E-mail：gzzsjzhs@163．com E-mail:shengshujing@foxmail．com
  • About author:生书晶（1981-），女，博士生，主要从事中药次生代谢途径研究．
  • Supported by:

    广东省自然科学基金资助项目（06019716）

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Abstract:

In order to regulate the secondary metabolic pathway of a Chinese traditional medicinal material Fallopia multiflora by genetic engineering technology,a gene named FmPKS2（GenBank accession number： GQ984139） was cloned via the rapid amplification of complementary DNA end and bioinformatics analysis.The gene consists of 1498bp nucleotides for coding sequence,encodes 392 amino acids with a predicted relative molecular mass of 43160 and an isoelectric point of 5.85,and contains several highly hydrophobic regions with a hydrophobic coefficient of-0.154.Phylogenetic analysis reveals that FmPKS2 shares the closed relationship with chalcone synthase of polygonaceae family.The transcription of FmPKS2 can be found in all different tissues of Fallopia multiflora,with the highest quantity in rhizomes.

Key words: Fallopia multiflora, polyketide synthase, cloning, expression, bioinformatics