Study on Beneficial Characteristics inVitro of Enterococcus Faecalis EF-ZA1107-06

doi:10.12141/j.issn.1000-565X.220798

Abstract

Abstract:

Studies have shown that probiotic has good antidiabetic and antioxidant activities and great inhibitory effects on human cancer cell lines. This paper took α-glucosidase and α-amylase inhibition rate, superoxide anion radical scavenging rate, ferrous ion chelating rate and reducing activity as indexes, and compared the antidiabetic and antioxidant capacities in vitro between Enterococcus faecalis EF-ZA1107-06 and Lactobacillus rhamnosus GG (LGG). The effects of EF-ZA1107-06 on cellular oxidative stress were investigated by measuring the enzyme activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and catalase (CAT) in Caco-2 cell lines. The anticancer activity and mechanism were also preliminarily explored using HepG2 and MDA-MB-231 cell lines. The results show that the supernatant of EF-ZA1107-06 has the highest scavenging rate of superoxide anion free radical from 40.78% to 59.61% and strongest ferrous ion chelating ability from 39.28% to 56.59%, while the lysate of EF-ZA1107-06 has the highest reducing activity, equivalent to 1.072 mmol/L equivalent cysteine, which is remarkably higher than that of LGG lysate (P<0.05). The inhibition of α-amylase and α-glucosidase activity confirms the antidiabetic activity of EF-ZA1107-06. Meanwhile, EF-ZA1107-06 can reduce oxidative damage caused by H₂O₂ to Caco-2 cells to a certain extent and inhibit the S phase and G2 phase of HepG2 cells, but only the S phase of MDA-MB-231 cells. The induction of the early apoptosis of HepG2 cells and MDA-MB-231 cells confirms the anticancer activity of EF-ZA1107-06.

Key words: Enterococcus faecalis, antioxidant capacity, anticancer activity, antidiabetic activity

CLC Number:

Q939.97

XU Xilin, PENG Yunyan, ZHOU Xiaoli, et al. Study on Beneficial Characteristics inVitro of Enterococcus Faecalis EF-ZA1107-06[J]. Journal of South China University of Technology(Natural Science Edition), 2023, 51(12): 118-130.

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组别	PNPG用量	样品用量	PBS用量	α-葡萄糖苷酶用量	Na₂CO₃用量
样品管（A）	1.0	1.0	1.0	1.0	10.0
样品空白管（B）	1.0	1.0	2.0	0.0	10.0
对照管（C）	1.0	0.0	2.0	1.0	10.0
空白管（D）	1.0	0.0	3.0	0.0	10.0

组别	样品用量	α-淀粉酶用量	PBS用量	2%可溶性淀粉用量	DNS用量	蒸馏水用量
样品管（A）	0.7	1.0	0.3	0.5	1.0	10.0
样品空白管（B）	0.0	1.0	1.0	0.5	1.0	10.0
对照管（C）	0.7	0.0	1.3	0.5	1.0	10.0
空白管（D）	0.0	0.0	2.0	0.5	1.0	10.0

菌株	菌株组分	相当于L-半胱氨酸的量/（mmol·L^-1）
EF-ZA1107-06	上清液	0.992^a
	菌悬液	0.169^b
	裂解液	1.072^a
LGG	上清液	1.106^a
	菌悬液	1.024^a
	裂解液	0.974^b

组别	GSH-Px酶活/（U·μg^-1）	SOD酶活/（U·μg^-1）	CAT酶活/（U·μg^-1）
空白组	2.15±0.00^c	23.74±1.02^a	26.57±0.41^a
损伤组	13.62±0.04^a	21.76±0.73^b	9.39±0.29^c
A1107-06上清液组	3.23±0.04^c*	22.83±0.92^a*	14.88±0.31^b*
LGG上清液组	7.71±0.75^b*	23.04±1.02^a*	17.48±0.47^b*
A1107-06灭活菌体组	1.62±0.08^c*	22.46±0.69^a*	15.19±0.50^b*
LGG灭活菌体组	4.31±0.52^bc*	22.36±1.07^a*	11.22±0.25^c
A1107-06裂解液组	4.83±0.52^bc*	22.77±1.30^a*	27.08±0.26^a*
LGG裂解液组	7.53±0.52^b*	23.17±1.35^a*	10.76±0.19^c