Safety Evaluation and Probiotic Characteristics of Enterococcus faecalis EF-ZA1107-06

doi:10.12141/j.issn.1000-565X.210731

Abstract

Abstract:

Enterococcus was isolated from 1-month-old baby feces in Guangzhou and the strain was identified as Enterococcus faecalis by molecular biology and was named Enterococcus faecalis EF-ZA1107-06. The strain was evaluated for its safety and some probiotic potential properties. The results show that the strains does not produce nitroreductase and has no hemolytic activity. It is highly sensitive to many kinds of antibiotics, does not contain plasmids, and has no acute toxicity to the mice. EF-ZA1107-06 was proved as an edible safe strain. High tolerance was observed by EF-ZA1107-06 strain for simulated gastrointestinal condition. After continuous exposure to simulated saliva for 10 min, simulated gastric juice for 2 h, and simulated intestinal juice for 3 h, the survivability was still above 65%, which was comparable to that of Lactobacillus rhamnosus GG (LGG). EF-ZA1107-06 can inhibit the growth of Escherichia coli, Salmonella, Pseudomonas aeruginosa, Candida albicans, Listeria monocytogenes, Staphylococcus aureus. The inhibitory effect of its supernatant on Escherichia coli is significantly stronger than that of LGG. The supernatant, lysate and suspension of the EF-ZA1107-06 strain all have certain DPPH free radical scavenging capacity and hydroxyl radical scavenging ability. DPPH free radical scavenging rate of 2×10⁸ CFU/mL EF-ZA1107-06 suspension is（93.16±0.17）%. The hydroxyl radical scavenging rate of the suspension is（90.48±1.12）%. The cholesterol removal rate of 2×10⁸ CFU/mL EF-ZA1107-06 fermentation broth is （44.29±0.30）%. The EF-ZA1107-06 strain has high cholesterol scavenging ability.

Key words: Enterococcus faecalis EF-ZA1107-06, tolerance of gastrointestinal condition, safety evaluation, probiotic characteristic, DPPH free radical scavenging capacity, hydroxyl radical scavenging ability, cholesterol scavenging efficiency

CLC Number:

Q939.97

XU Xilin, ZHONG Shuying, ZHOU Xiaoli, et al. Safety Evaluation and Probiotic Characteristics of Enterococcus faecalis EF-ZA1107-06[J]. Journal of South China University of Technology(Natural Science Edition), 2022, 50(11): 52-61.

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药敏纸片名称	药物含量/μg	抑菌圈直径判断标准/mm
药敏纸片名称	药物含量/μg	R	I	S
环丙沙星	5	≤15	16-20	≥21
氧氟沙星	5	≤12	13-15	≥16
利福平	5	≤16	17-19	≥20
美罗培南	10	≤19	20-22	≥23
头孢唑林	30	≤14	—	≥15
头孢呋辛	30	≤14	15-17	≥18
卡那霉素	30	≤13	14-17	≥18
四环素	30	≤14	15-18	≥19
氯霉素	30	≤12	13-17	≥18
青霉素	10	≤14	—	≥15
万古霉素	30	≤14	15-16	≥17

药敏片	Enterococcus faecalis EF-ZA1107-06抑菌圈直径/mm	敏感度	Staphylococcus aureusATCC12592 抑菌圈直径/mm	敏感度
环丙沙星	16.3±0.41	I	19.1±0.05	I
氧氟沙星	16.3±1.24	S	17.8±1.25	S
利福平	17.2±0.23	I	18.3±0.75	I
美罗培南	19.7±1.69	I	20.0±1.00	I
头孢唑林	16.0±0.82	S	22.0±1.50	S
头孢呋辛	19.0±0.00	S	22.2±0.15	S
四环素	23.3±0.47	S	20.3±1.65	S
氯霉素	22.2±0.85	S	26.5±0.50	S
青霉素	20.7±1.84	S	25.3±0.25	S
万古霉素	16.7±0.94	S	19.7±0.24	S

动物性别	剂量/（g·kg^-1）	动物数/只	体质量（X±SD）/g			死亡数/只	死亡率/%
动物性别	剂量/（g·kg^-1）	动物数/只	0 d	7 d	14 d	死亡数/只	死亡率/%
雌性	10.0	10	19.90±0.64	30.77±1.33	34.85±1.47	0	0
雄性	10.0	10	20.73±1.37	36.06±1.97	42.32±3.26	0	0

菌株	存活率/%
菌株	人工唾液	人工胃液	人工肠液
EF-ZA1107-06	97.3±0.19^a	79.4±0.16^b	67.2±0.06^a
LGG	93.6±0.01^b	83.4±0.24^a	68.9±0.15^a

指示菌	抑菌圈直径/mm
	EF-ZA1107-06			LGG
	菌悬液		上清液	菌悬液	上清液
大肠杆菌	16.5±0.08^c	20.5±1.00^b		23.3±0.12^a		14.5±0.07^d
沙门氏菌	16.0±1.00^c	16.7±0.03^c		26.0±0.01^a		20.6±0.09^b
铜绿假单胞菌	10.3±0.25^d	15.0±1.00^c		21.7±0.08^a		19.2±0.12^b
白色念珠菌	10.2±0.12^d	15.3±0.25^c		24.1±0.06^a		22.0±0.01^b
单增李斯特菌	13.0±1.00^c	17.3±1.06^ab		20.0±0.07^a		16.7±0.08^b
金黄色葡萄球菌	16.0±0.20^d	16.7±0.02^c		21.3±0.05^a		17.3±0.12^b