Journal of South China University of Technology (Natural Science Edition) ›› 2017, Vol. 45 ›› Issue (3): 146-152.doi: 10.3969/j.issn.1000-565X.2017.03.021

• Food Science & Technology • Previous Articles    

Investigation into Identification，Antioxidant Activity and Anti-Hemolysis Activity of Phenolics Compounds in High Acid Apple

SUN Wei-zheng YANG Kun LIN Lian-zhu ZOU Ying ZHAO Mou-ming   

  1. School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2016-05-04 Revised:2016-08-29 Online:2017-03-25 Published:2017-02-02
  • Contact: 赵谋明( 1964-) ，男，博士，教授，主要从事食品生物技术研究． E-mail:femmzhao@scut.edu.cn
  • About author:孙为正( 1983-) ，男，博士，教授，主要从事食品蛋白质与营养研究． E-mail: fewzhsun@ scut． edu． cn
  • Supported by:
    Supported by the Dr． Start-up Fund Projects of the Natural Science Foundation of Guangdong Province ( 2015A030310494) and the Special Support Project of Guangdong Province for Science and Technology Innovative Young Talents ( 2014TQ01N538 )

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Abstract:

The composition of a kind of high acid apple from Xinjiang,China,namely Hongxun Ⅰ ( red flesh) ,was determined,finding that the high acid apple is of a titratable acidity of 1. 31%,a total phenolics and anthocyanins content in an order of peel ＞ pomace ＞ whole fruit ( with a total phenolics content of ( 2026. 88 ±28. 30) mg /kg and a total anthocyanins content of ( 244. 92 ± 13. 88) mg /kg) ＞ flesh.Then,ten phenolic compounds including chlorogenic acid,phlorizin,epicatechin,ferulic acid,myricetin,catechins,vanillic acid,p-coumaric acid,rutin and caffeic acid were indentified by means of high-performance liquid chromatography,finding that the high acid apple is rich in chlorogenic acid ( ( 391. 19 ± 18. 37) mg /kg) ,epicatechin ( ( 698. 82 ± 17. 21) mg /kg) and catechins ( ( 570. 30 ± 9. 21) mg /kg) .Moreover,the antioxidant and anti-hemolysis activities of the high acid apple were determined by means of DPPH radical scavenging activity assay,oxygen radical absorption capacity assay and AAPH-induced oxidative damage human erythrocytes model,finding that the peel,flesh,pomace and whole fruit of the high acid apple all exhibit excellent antioxidant activity and that the anti-hemolysis activities are indicative of the following order: peel ＞ pomace ＞ whole fruit ＞ flesh.All these above-mentioned statements indicate that,as a valuable product,high acid apple is worth processing and developing.

Key words: high acid apple, antioxidant activity, anti-hemolysis activity, phenolics