Journal of South China University of Technology (Natural Science Edition) ›› 2013, Vol. 41 ›› Issue (10): 47-52,65.doi: 10.3969/j.issn.1000-565X.2013.10.008

• Food Science & Technology • Previous Articles     Next Articles

Fed-Batch Culture of High Cell-Density Oleaginous Yeast Trichosporon fermentans HWZ004

Zong Min-hua1,2 Liu Zong-jun3 Wu Hong1,2,4 Lou Wen-yong1,2 Zhu Ding-he2,4   

  1. 1.State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;2.School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China;3.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;4.Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,South China University ofTechnology,Guangzhou 510640,Guangdong,China
  • Received:2013-04-01 Revised:2013-05-20 Online:2013-10-25 Published:2013-09-03
  • Contact: 吴虹(1971-)，女，博士，副教授，主要从事油脂化工研究． E-mail:bbhwu@scut.edu.cn
  • About author:宗敏华(1960-)，女，博士，教授，主要从事生物化工研究.E-mail:btmhzong@scut．edu．cn
  • Supported by:

    国家自然科学基金资助项目( 31071559) ; 教育部新世纪优秀人才支持计划项目( NCET-11-0161，NCET-10-0367) ;制浆造纸工程国家重点实验室开放基金资助项目( 201138) ; 广东省天然产物绿色加工与产品安全重点实验室开放基金资助项目( 201207)

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Abstract:

In order to improve the ability of oleaginous yeast Trichosporon fermentans HWZ004 to produce lipid，afed-batch culture with high cell density was carried out.The effect of the initial sugar concentration on the growthand lipid accumulation of T. fermentans HWZ004 was investigated and the initial sugar concentration was determinedas 100 g /L,at which T. fermentans HWZ004 could achieve more biomass and higher lipid yield without substrateinhibition. After a 132 h fed-batch culture in a 5-L fermentor,it is found that the biomass,lipid content andlipid productivity of T.fermentans HWZ004 are respectively 102 g /L,48.3% and 0.37 g / ( L·h) .GC results revealthat the fatty acids in the obtained lipid are mainly stearic acid,palmitic acid,oleic acid and linoleic acid，and the unsaturated fatty acid content is over 65%,which means that the lipid is suitable for the biodiesel production.

Key words: Trichosporon fermentans HWZ004, fed-batch fermentation, high-density cultivation, biomass, lipid yield

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