Journal of South China University of Technology (Natural Science Edition) ›› 2013, Vol. 41 ›› Issue (10): 40-46.doi: 10.3969/j.issn.1000-565X.2013.10.007

• Food Science & Technology • Previous Articles     Next Articles

Relative Molecular Mass and Concentration Analyses of Sugarcane Dextran in Mixed Juice with Enzymatic Hydrolysis

Zhao Zhen-gang1,2 He Xiang2 Yu Shu-juan1,2   

  1. 1.Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,Guangzhou 510640,Guangdong,China; 2.School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2012-08-22 Revised:2012-12-09 Online:2013-10-25 Published:2013-09-03
  • Contact: 于淑娟(1955-)，女，教授，博士生导师，主要从事功能性碳水化合物研究． E-mail:lfshjy@scut.edu.cn
  • About author:赵振刚(1979-)，男，博士，讲师，主要从事功能性碳水化合物研究．E-mail:fezzg@scut．edu．cn
  • Supported by:

    国家“十二五”科技支撑计划项目( 2011BAE16B04) ; 广东省天然产物绿色加工与产品安全重点实验室开放课题( 201104) ; 广东省自然科学基金博士启动项目( S2011040000384) ; 广东省教育厅育苗工程项目( LYM11017)

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Abstract:

The crude dextran was separated and purified from the mixed juice ( MJ) of sugarcane through ethanolprecipitation,enzymatic hydrolysis and ultrafiltration,and the corresponding relative molecular mass distributionwas characterized by means of GPC. Then,dextranase was used to hydrolyze the dextran in the MJ,and the changelaws of the relative molecular mass and concentration of dextran during the enzymatic hydrolysis were investigated bymeans of GPC and IC.The results show that (1) the relative molecular masses of the dextran in the MJ stored atthe room temperature for 20h are mainly 1.02 × 106 and 3.57 × 103 ; (2) the relative molecular mass of the dextransignificantly decreases with the extension of the reaction time; (3) dextranase can hydrolyze the dextran into theoligosaccharides，which are the final major products; and (4) the concentration change of dextran is related to thecorresponding relative molecular mass change.

Key words: mixed juice, dextranase, dextran, relative molecular mass

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