Journal of South China University of Technology (Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (5): 160-164.doi: 10.3969/j.issn.1000-565X.2011.05.028

• Biological Engineering • Previous Articles    

Preparation and Application of Dextran-Ovomucoid Affinity Ultrafiltration Carrier

Li Cun-zhi1  Huang Xue-song1  Tang Shu-ze1  Li Lin2  Zhang Guang-wen1  Hu Chang-ying1   

  1. 1.Department of Food Science and Engineering,Jinan University,Guangzhou 510632,Guangdong,China;2.School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2011-01-29 Revised:2011-03-11 Online:2011-05-25 Published:2011-04-01
  • Contact: 李琳(1963-)，男，博士，教授，主要从事糖类物质及其药物的制备与生物利用研究．E-mail: felinl@scut．edu．cn E-mail:felinl@scut.edu.cn
  • About author:李存芝(1969-)，女，博士，讲师，主要从事食品化学、食品加工研究
  • Supported by:

    国家自然科学基金资助项目(30871751)；暨南大学博士科研启动基金资助项目(51205012)

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Abstract:

In order to reveal the preparation mechanism of dextran-ovomucoid affinity ultrafiltration carrier and evaluate the application effect of the carrier,first,dextran T2000 ( dextran with a relative molecular mass of 2 × 106 ) was employed to synthesize a water-soluble affinity carrier after the epichlorohydrin activation,with ovomucoid as the affinity ligand. Then,the changes of infrared adsorption spectrum and ultraviolet adsorption spectrum of the affinity carrier during the preparation were investigated,and pilot study on trypsin purified by the affinity ultrafiltration was performed. The results show that the activation of dextran can be described as a bimolecular nucleophilic substitution ( SN2) process,that the adsorption isotherm of trypsin by dextran-ovomucoid carrier can be described by the Langmuir isotherm equation,that the purification factor and the activity recovery rate respectively reach 45 times and 80%,and that the affinity of the carrier to trypsin is of high specificity,which may result in excellent purification effect of trypsin by affinity ultrafiltration.

Key words: affinity ultrafiltration, dextran, ovomucoid, epichlorohydrin, trypsin

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