Journal of South China University of Technology(Natural Science Edition) ›› 2023, Vol. 51 ›› Issue (12): 152-158.doi: 10.12141/j.issn.1000-565X.220145

• Food Science & Technology • Previous Articles    

Extracting Oil from Diacylglycerol Microcapsules with Bromelain

LAN Dongming1 WAN Chufeng1 CHEN Ying1 LIU Xuan1 LUO Riming2 WANG Weifei3 WANG Yonghua1,4   

  1. 1.School of Food Science and Engineering，South China University of Technology，Guangzhou 510640，Guangdong，China
    2.Guangdong Yueshan Specialty Nutrition Technology Co. ，Ltd. ，Foshan 528226，Guangdong，China
    3.Guangdong Academy of Agricultural Science，Guangzhou 510640，Guangdong，China
    4.Guangdong Youmei Bio-Manufacturing Research Institute，Foshan 528226，Guangdong，China
  • Received:2022-05-05 Online:2023-12-25 Published:2022-05-06
  • About author:蓝东明（1980-），男，副研究员，博士生导师，主要从事新型（食品）酶的挖掘、结构功能关系及应用研究。E-mail： dmlan@scut.edu.cn
  • Supported by:
    the National Science Foundation for Distinguished Young Scholars(31725022);the National Natural Science Foundation of China(31930084);the Science and Technology Project of Guangdong Province of China(2019A050503002);the Agricultural Research System of China(CARS-18-ZJ0503);the Science and Technology Innovation Project of Foshan of China(FS0AA-KJ919-4402-0013)

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Abstract:

Diacylglycerol microcapsule is a new type of functional food ingredient. In order to accurately determine the oil content, peroxide value, diacylglycerol content and other physical and chemical indexes of diacylglycerol microcapsule, the optimal extraction method for the oil components should be established. In this study, four methods of acid hydrolysis, alkali hydrolysis, ultrasonic treatment and bromelain hydrolysis were used for wall-breaking pretreatment of flaxseed diacylglycerol microcapsule. The effects of the different pretreatment methods on the extraction of fats and oils from the microcapsules were compared. In other words, the extraction rate of the fats and oils and the peroxide value of the them were investigated, and the glyceride composition of the extracted fats and oils was analyzed by high performance liquid chromatography. The results show that: the diacylglycerol obtained by the bromelain hydrolysis method has the least loss and the lowest peroxide value; the diacylglycerol obtained by acid hydrolysis and ultrasonic treatment has higher extraction rate, but acid hydrolysis results in the greatest loss of diacylglycerol content and ultrasonic treatment results in the highest peroxide values; and the alkali hydrolysis treatment leads to the lowest oil extraction rate. On the basis of the single factor experiment of enzyme hydrolysis, orthogonal optimization test was performed to explore the effect of enzyme addition, temperature and time on oil extraction rate, and the extraction solvent after enzymatic digestion was also optimized. The results show that when the dosage of bromelain is 12 000 U/g, the enzymatic hydrolysis temperature is 55 ℃, and the enzymatic hydrolysis time is 5 min, the oil extraction rate of the diacylglycerol microcapsules can reach (98.60±0.78)%, and the peroxide value of the extracted oil is (0.037±0.001) g/100 g. The optimal extraction system after enzymatic hydrolysis is ethanol-diethyl ether-petroleum ether (2:5:5, in volume ratio). This method has high oil extraction rate and little impact on oil quality, providing theoretical basis for the detection of physicochemical properties of diacylglycerol microcapsules.

Key words: diacylglycerol, microcapsule, oil extraction, orthogonal design

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