Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (9): 32-37.

• Chemistry & Chemical Engineering • Previous Articles     Next Articles

Simultaneous Determination of Stilbene Glucoside and Five Anthraquinones in Polygonum multiflorum Thunb. by Means of Micellar Electrokinetic Capillary Chromatography

Wu Hong  Wang Yan  Wen Peng  Lou Wen-yong  Zong Min-hua   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2012-03-26 Revised:2012-06-27 Online:2012-09-25 Published:2012-08-01
  • Contact: 吴虹(1971-) ，女，博士，副教授，主要从事生物化工研究． E-mail:bbhwu@scut.edu.cn
  • About author:吴虹(1971-) ，女，博士，副教授，主要从事生物化工研究．
  • Supported by:

    国家自然科学基金资助项目( 31071559) ; 教育部新世纪优秀人才支持计划项目( NCET-11-0161)

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Abstract:

In this investigation,a method of using the micellar electrokinetic chromatography was laid down for the simultaneous determination of stilbene glucoside and five anthraquinones,including emodin,aloe-emodin,rhein,chrysophanol and physcion,in Polygonum multiflorum Thunb.,and the effects of the buffer solution pH value,the SDS concentration,the sodium tetraborate concentration,the methanol volume fraction and the operating voltage on
the separation and determination were discussed. The results show that optimal separation and determination conditions are an operating voltage of 25 kV and a buffer solution with a pH value of 9.6,15mmol /L of sodium tetraborate,30mmol /L of SDS and 10%( volume fraction) of methanol,and that, in the optimal conditions,stilbene glucoside and the five anthraquinones are completely separated within 18minutes,with a relative standard deviation of migration times of 1.06% ～ 1.61%,a relative standard deviation of peak areas of 1.46% ～ 2.87%,a detection limit of 0.26 ～ 0.56mg /L and a recovery of 97.2% ～ 103.2%.

Key words: micellar electrokinetic capillary chromatography, Polygonum multiflorum Thunb., stilbene glucoside, anthraquinones

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