Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (5): 90-95.

• Biological Engineering • Previous Articles     Next Articles

Construction of Engineering Escherichia coli for Production of Coenzyme Q10

Li Jia-zhou1  Xie Ming-quan1  Li An-xing2  Luo Xiao-chun1   

  1. 1. School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China; 2. Institute of Aquatic Economic Animals,Sun Yat-Sen University,Guangzhou 510275,Guangdong,Ch
  • Received:2011-10-09 Revised:2012-02-15 Online:2012-05-25 Published:2012-03-31
  • Contact: 罗晓春(1977-) ，男，副教授，主要从事分子生物学研究． E-mail: xcluo@scut．edu．cn E-mail:2003102021@gditc.edu.cn
  • About author:李家洲(1976-) ，男，博士生，副教授，主要从事微生物分子生物学和微生物工程研究．
  • Supported by:

    广东省自然科学基金资助项目( 7004113) ; 广东省工业攻关项目( 2011B010500018)

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Abstract:

In this paper,decaprenyl diphosphate synthase ( ddsA) gene from Rhizobium radiobacter was cloned and used to construct a target fragment for red recombination. Then,the ispB gene in Escherichia coli genome was substituted with ddsA gene by homologic recombination,which made the recombinant Escherichia coli be capable of synthesizing CoQ10 instead of CoQ8 . Moreover,some key enzyme genes for CoQ synthesis,such as ddsA,ubiA,ispA and idi,were strengthened to improve the production of CoQ10 ( up to 126. 9%) . Comprehensive analyses show that ddsA and ubiA are key genes,while ispA and idi are secondary key genes for CoQ10 synthesis.

Key words: coenzyme Q, Escherichia coli, ddsA gene, engineering bacteria, homologous recombination

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