Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (5): 149-154.

• Food Science & Technology • Previous Articles    

Isolation of Campylobacter Strains in Poultry Products and Genotyping Identification of Strains by Means of PFGE and DGGE

Meng He-cheng1  Bi Shui-lian2   Yan He1,3  Shi Lei1   

  1. 1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China; 2. School of Food Science,Guangdong Pharmaceutical University,Zhongshan 528435,Guangdong,China; 3. Food Safety Key Lab of Liaoning Province,Jinzhou 121013,Liaoning,China
  • Received:2011-12-20 Revised:2012-03-01 Online:2012-05-25 Published:2012-03-31
  • Contact: 石磊(1961-) ，男，博士，教授，主要从事病原微生物快速诊断和耐药机制研究． E-mail: leishi@scut．edu．cn E-mail:arsenal0001@ hotmail.com
  • About author:孟赫诚(1984-) ，男，博士，主要从事微生物快速诊断和分子耐药研究．
  • Supported by:

    广东省自然科学基金资助项目( 10451064101005159) ; 华南理工大学中央高校基本科研业务费专项资金资助项目( 2012ZZ0083) ; 辽宁省食品安全重点实验室开放课题资助项目( LNSAKF2011002)

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Abstract:

Eight poultry samples including chicken and duck were collected in a certain fresh market and were enriched in Bolton and Preston broths,followed by the inoculation on selective agar media ( Skirrow and mCCDA) and by the membrane filtration. Then,the molecular types of the isolated Campylobacter strains were analyzed by means of pulse field gel electrophoresis ( PFGE) and denaturing gradient gel electrophoresis ( DGGE) ,and C. jejuni or C. coli were isolated from six samples. The results show that the combination of Preston broth and mCCDA media is the optimal isolation method,that there are four samples contaminated by the same C. coli isolate and two samples contaminated respectively by 2 species of C. jejuni and 3 species of C. coli,and that,though DGGE genotyping is not as sensitive as PFGE genotyping,it is more suitable for the rapid genotyping of Campylobacter in food borne because it is of high speed,low cost as well as stable and accurate operation.

Key words: Campylobacter, isolation, genotyping identification, pulse field gel electrophoresis, denaturing gradient gel electrophoresis