Journal of South China University of Technology(Natural Science Edition) ›› 2012, Vol. 40 ›› Issue (5): 139-144.

• Food Science & Technology • Previous Articles     Next Articles

Effects of Fractionation by Alcohol Precipitation and Deproteinization by Enzyme on Properties of Beet Dietary Fibre

Zhu Si-ming  Wang Wen-ting  Yu Shu-juan   

  1. Research Institute of Light Industry and Chemical Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2012-02-16 Online:2012-05-25 Published:2012-03-31
  • Contact: 于淑娟(1955-),女,教授,博士生导师,主要从事制糖工程研究.E-mail:lfshjyu@scut.edu.cn E-mail:lfsmzhu@ scut.edu.cn
  • About author:朱思明(1976-) ,男,博士,助理研究员,主要从事糖厂综合利用研究.
  • Supported by:

    国家科技支撑计划项目( 2011BAE16B02) ; 广东省科技计划项目( 2010A080403005) ; 华南理工大学中央高校基本科研业务费专项资金资助项目( 2012ZM0072)

Abstract:

In order to reveal the influences of extraction and purification on the properties of beet dietary fibre and promote the value increment of beet pulp,the effects of beet pulp materials and pretreatment method on the quality of beet pectin were discussed. Then,the physiochemical properties of the beet pectin graded by acid extraction and alcohol precipitation were analyzed by using a HPLC and a Haake rheometer,and the morphological and structural changes of the insoluble dietary fibre ( IDF) after the deproteinization by enzymes were investigated by means of SEM. The results indicate that the pretreatment with dilute acid or warm water is unnecessary for the extraction of pectin from 3 kinds of beet pulp,that the product of yield and purity reaches the maximum in the extraction of pectin from grain pulp under optimal conditions,and that the beet pectin graded by acid extraction and alcohol precipitation includes a suspended layer and a deposited layer,and that the suspended pectin layer is of lower density,lighter color,higher viscosity and greater percentage of large molecules. Moreover,it is found that,after the pectin extraction,the cavernous skeleton structure of the pressed beet pulp becomes smoother and the filler particles in cavity disappear,and that further deproteinzation treatment enlarges the cavity and results in the lamellar skeleton structure.

Key words: beet dietary fibre, fractionation, deproteinization, alcohol precipitation, rheological behaviour, skeleton structure