Journal of South China University of Technology(Natural Science Edition) ›› 2011, Vol. 39 ›› Issue (12): 110-114.

• Food Science & Technology • Previous Articles     Next Articles

Composition and Antitumor Activity of Polysaccharides from Sarcodon imbricatus

Chen Jian  Zhang Ling-zhi  Wei Ding  Xu Xiao-fei   

  1. School of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Guangdong,China
  • Received:2011-04-29 Revised:2011-09-21 Online:2011-12-25 Published:2011-11-04
  • Contact: 陈健(1967-) ，男，博士，副教授，主要从事天然产物化学和生物分离技术研究． E-mail:fejchen@ scut.edu.cn
  • About author:陈健(1967-) ，男，博士，副教授，主要从事天然产物化学和生物分离技术研究．
  • Supported by:

    广东省科技计划项目( 2009B011300002)

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Abstract:

Two kinds of polysaccharides,namely SIPa and SIPb,were obtained from the fruit body of Sarcodon imbricatus via isolation and purification. Then,the IR spectra of the products were investigated,and the relative molecular mass and monosaccharide composition of each product were determined by means of high-performance gel permeation chromatography and gas chromatography. Moreover,the antitumor activities of the two products were
evaluated by means of the MTT method. The results show that ( 1) SIPa and SIPb both possess typical infrared absorptions of polysaccharides; ( 2) the sugar chain of SIPa is mainly β-configuration pyranoside; ( 3) the weightaverage relative molecular mass of SIPa and SIPb are respectively 2. 12 × 105 and 1. 05 × 104 ; ( 4) SIPa is a kind of glucan while SIPb is a kind of heteroglycan containing fucose,mannose,glucose and galactose in a molar ratio of 8. 93: 1: 4. 25: 29. 18; and ( 5) both SIPa and SIPb remarkably inhibit the growth of human liver cancer cell line Hep G2 and human ovarian cancer cell line HO-8910 in a dose-dependent manner.

Key words: Sarcodon imbricatus, polysaccharides, monosaccharide composition, antitumor activity