Journal of South China University of Technology(Natural Science Edition)

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 Effects of Extraction Techniques on Anticoagulant Activity of Acid-Auricularia auricular Polysaccharides
 

 LI Dehai1 GU Jialin1 SUN Changyan2† XU Ying1 ZONG Fangfang1   

  1.  1． College of Forestry，Northeast Forestry University，Harbin 150040，Heilongjiang，China; 2． Department of Food Science and Engineering，School of Chemical and Environmental Engineering，Harbin University of Science and Technology，Harbin 150080，Heilongjiang，China
  • Received:2016-05-25 Online:2018-06-25 Published:2018-05-07
  • Contact: 孙常雁( 1980-) ，女，博士，高级工程师，主要从事食品化学研究 E-mail:scyneau@163.com
  • About author:李德海( 1976-) ，男，博士，副教授，主要从事食品化学及植物有效成分研究
  • Supported by:
     Supported by the Natural Science Foundation of Heilongjiang Province( C2015062) and the Fundamental Ｒesearch Funds for the Central Universities( 2572014CA19) 

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Abstract:  The inhibition of fibrinogen ( FIB) to fibrin， the activated partial thromboplastin time ( APTT) ， the prothrombin time ( PT) and the thrombin time ( TT) were used to study the anticoagulant activity of acid-Auricularia auricular polysaccharides which were obtained by three kinds of physical-assisted extraction techniques，ultrasound，microwave and high shear． And then the correlation between the extraction technique and the anticoagulant activity was analyzed by testing the structures and physicochemical properties of polysaccharides． The results showed that the acid-Auricularia auricula polysaccharides had a significant inhibitory effect on the transformation of FIB，and it could also prolong the APTT and TT of rabbit plasma ( p ＜0. 05) significantly，but it produced no significant effect on PT ( p ＞0. 05) ，so it is through endogenous pathway and common pathway to involve itself in the coagulation process． The polysaccharides with the best anticoagulant activity in each treatment were selected for further comparative analysis，and the W-Ac-AAP，which was obtained by microwave-assisted extraction for 15 min ( W-Ac-AAP) ，had the best anticoagulant activity． When the concentration of polysaccharide at 4. 0 g/L，the APTT was ( 125. 03 ±1. 64) s and the TT was ( 70. 39 ±0. 87) s． When the concentration of polysaccharide at 2. 0 g/L， the ability of FIB to inhibit fibrin conversion was ( 0. 76 ±0. 05) g/L． There had been significant differences in protein content and sulfate content among the four polysaccharides ( p ＜ 0. 05) ． The W-Ac-AAP with the best anticoagulant activity had the lowest protein content， the highest sulfate content，and polysaccharides with common alkali extraction ( Ac-AAP) had the lowest sulfate content， there would be a certain correlation between the anticoagulant activity and sulfate content of polysaccharides． This study provides a means for the development of natural plant anticoagulant drugs． 

Key words: acid-Auricularia auricular polysaccharides, extraction techniques, anticoagulant activity, physicochemcial property

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