Journal of South China University of Technology (Natural Science Edition) ›› 2010, Vol. 38 ›› Issue (8): 51-55.doi: 10.3969/j.issn.1000-565X.2010.08.010

• Chemistry & Chemical Engineering • Previous Articles     Next Articles

Enzymatic Properties of Urease Immobilized on Enzymic Dialdehyde Starch

Luo Zhi-gang 1.2  Yang Jing-feng3  Wang Min-ni 2   

  1. 1. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China; 2. Carbohydrate Laboratory, Research Institute of Light Industry and Chemical Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China; 3. Research Institute of Agricultural Science and Technology Information, Guangxi Academy of Agricuhural Sciences, Nanning 530007, Guangxi, China
  • Received:2009-09-03 Revised:2010-01-26 Online:2010-08-25 Published:2010-08-25
  • Contact: 罗志刚（1975-），男，博士，副教授，主要从事碳水化合物化学与工程研究． E-mail:zhgluo@scut．edu．cn
  • About author:罗志刚（1975-），男，博士，副教授，主要从事碳水化合物化学与工程研究．
  • Supported by:

    高等学校博士点专项科研基金资助项目（20070561078）;广东省产学研结合项目（20078090400003,20098090300272）;广东省科技攻关项目（20088021100016,20098020312006）;华南理工大学中央高校基本科研业务费资助项目（2009ZM0124）

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Abstract:

This paper deals with the immobilization and properties of urease on enzymic dialdehyde starch. SEM results show that, as a carrier, enzymic dialdehyde starch aggregates after the immobilization. Moreover, it is found that （ 1 ） the content of urease immobilized on enzymic dialdehyde starch is up to 24.3 % ; （2） the optimal pH value and temperature for the immobilization are respectively 6. 0 and 70 ℃ ; （3） the immobilized urease is of excellent storage stability and large Michaelis constant; and （4） the activity of immobilized urease decreases only by 25% after being used for ten times. It is thus concluded that enzymic dialdehyde starch is an effective cartier for immobilized enzymes such as urease.

Key words: enzymic dialdehyde starch, urease, immobilization, property