Journal of South China University of Technology (Natural Science Edition) ›› 2008, Vol. 36 ›› Issue (4): 151-154.

• Biology Science • Previous Articles    

Analysis of 18S rRNA Gene Sequence of Polygonum multiflorum

Yan Ping1  Jiao Xu-wen1  Pang Qi-hua2  Zhao Shu-jin3    

  1. 1.School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China; 2. College of Life Science, South China Normal University, Guangzhou 510631, Guangdong, China; 3. General Hospital of Guangzhou Military Command, Guangzhou 510010, Guangdong, China
  • Received:2007-01-22 Revised:2007-05-15 Online:2008-04-25 Published:2008-04-25
  • Contact: 赵树进，教授，博士生导师． E-mail:gzzsjzhs@pub．guangzhou．gd．cn
  • About author:严萍（1976-），女，博士生，主要从事生物制药研究．E-mail：ycyyp22@hotmail．com
  • Supported by:

    广东省自然科学基金资助项目（6104397）

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Abstract:

In order to reveal the relationship between the cultivated and the wild Polygonum multifiorum from different geographical locations and to provide evidences for the molecular identification, the 18S rRNA gene sequences of wild Polygonum multiflorum Thunb, the cultivated Polygonum multiflorum and Musa Basjoo Sieb. ＆ Zucc were analyzed. The results show that the 18S rRNA gene sequences of Polygonum multiflorum are 1809bp in length and are highly conserved, and that nucleotide substitutions occur at positions 680, 1712 and 1724. As compared with Polygonum multijflorum sequences, Musa Basjoo Sieb. ＆Zucc is of 71 nucleotide substitutions, a T＆A deletion at positions 135 and 500 and a C＆A insertion at positions 284 and 1 537. According to the homology of 18S rRNA gene sequences, the cultivated and the wild Polygonum multiflorum have identical botanical origins, which means that DNA sequencing is an accurate multiflorum. and effective method of molecular identification of Polygonum

Key words: molecular identification, Polygonum multiflorum, gene, sequence analysis