Abstract:

In order to investigate the application of moderately halophilic bacterium to the treatment of hypersaline organic tanning wastewater, a halophilic bacterium YS-1 was isolated from the salt water of a salina. The atomic force microscopy （AFM） observation, the physiologic-biochemical index measurement, the whole-cell fatty acid analysis and the 16S rDNA sequence homology identification for the bacterium were then carried out. The results show that the closest relative of YS-1 strain is Halomonas sp. （AB167061）, meaning that YS-1 strain is a novel moderately halophilic species of Halomonas sp.. Moreover, according to the results of hypersaline tanning wastewater treatment by the identified bacterium combined with the biological activated carbon （BAC） technology in a sequencing batch reactor （SBR）, it is found that, for the wastewater with an initial salt content of 9.3% and an initial CODer of 1378mg,/L, the CODcr removal reaches 86% after the treatment for 168h, and rises to 98% after 216h. It is thus concluded that YS-1 strain can enhance the treatment of hypersaline tanning wastewater, and that a ＂halophilic-BAC technology＂ combining the screened bacterium and the BAC technology is feasible in the treatment of hypersaline tanning wastewater.

Key words: moderately halophilic bacterium, isolation, identification, hypersaline tanning wastewater, wastewater treatment