Journal of South China University of Technology (Natural Science Edition) ›› 2007, Vol. 35 ›› Issue (11): 125-128.

• Biotechnology • Previous Articles    

Synthesis of Artificial Morphine Antigen and Preparation of Polyclonal Anti-Morphine Antibody

He Xiao-wei1  Liu Xim-yun1  Yang Lian-sheng1  Peng Yun-ping2  Huang Qiang1  Wang Ji-hua2   

  1. 1. School of Light Industry and Food Sciences , South China Univ. of Tech. , Guangzhou 510640 ， Guangdong, China;2. Guangzhou Wondfo Biotech Co. , Ltd. , Guangzhou 510640 ， Guangdong, China
  • Received:2006-09-27 Online:2007-11-25 Published:2007-11-25
  • Contact: 何小维(1959-) ，男，博士，副教授，主要从事生物医疗高分子材料研究 E-mail:davidxwhe@126. com
  • About author:何小维(1959-) ，男，博士，副教授，主要从事生物医疗高分子材料研究
  • Supported by:

    国家自然科学基金资助项目(29774009); 广东省科技推广项目(2006B40101013)

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Abstract:

Artificial morphine antigen was chemically synthesized and was then used to immunize the New Zealand rabbits to prepare the polyclinal anti -morphine antibody. Mterwards , the morphine antigen is respectively coupled with two protein carriers( BSA and OV A) by means of mixed acid anhydride method. It is proved by UV scan and protein electrophoresis that the artificial morphine antigen is successfully synthesized. Moreover, it is proved by EUSA results that the rabbit serum with high dilute degree is prepared , and that there is a high-specificity reaction between the polyclonal antibody and the antigen. Both the antigen and the antibody can be applied to the research on morphine immunoassay.