Abstract:

A novel technology about the integration of separation and coordination reaction of hesperidin with Cu²⁺ in ion -exchange chromatography (IEC)column was presented and performed in this paper. Ion -Exchange Chromatography , UV -vis , XRD FTIR and ¹ H -NMR were adopted to analyze the mechanism of the technology. The results show that it is feasible to produce new medicines with high biological activity by the column reaction between metal ions (Cu^{2 +}) and Chinese medicine , such as hesperidin. The reaction mechanism lies in the fact that as a soft base , anion hesperidin on resin can easily coordinate with Cu²⁺ insolution due to the electrostatic attraction between them , which modifies the molecular state and hydrophobic state of hesperidin by the "centralizing effect" of Cu²⁺ on hesperetin and the "embedding effect" of rutinose on hesperetin. As a result , the anions in the regenerant go through the Nemst film to replace hesperidin , and hesperidin disperses in the liquidphase due to the overlapping of
electron clouds , thus contributing to the integration of separation and coordination reaction.

Key words: hesperidin-Cu²⁺ complex, separation, coordination reaction, integration, ion-exchange chromatography