Journal of South China University of Technology (Natural Science Edition) ›› 2006, Vol. 34 ›› Issue (5): 43-47,52.

• Biological Engineering • Previous Articles     Next Articles

Agrobacterium tumefaciens-Mediated Genetic Transformation of Roselle Cell

Xie Xiu-zhen  Lin Qiao-hui  Guo Cheng-shuan  Guo Yong   

  1. College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2005-07-12 Online:2006-05-25 Published:2006-05-25
  • Contact: 郭勇，教授，E—mail：btyguo@ scut．edu．cn E-mail:xiexiuzhen@sina.com
  • About author:谢秀祯(1960-)，男，在职博士生，海南师范大学教授，主要从事基因工程的研究
  • Supported by:

    广东省重点科技攻关项目(A301020201)

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Abstract:

By using Agrobacterium tumefaciens strains LBA4404 and EHA 105 as the donor strains.the cotyledon and hypocotyl of sterile seedling of roselle as the recipient materials.the basic conditions for the genetic transforma.tion of Roselle cell in vitro were investigated.Then a high-efficiency genetic transformation system was established ,by which two transgenic Roselle cell lines that stably express the activity of neomycin phosphotI_ans rase Ⅱ were ob tained.The results of β-glucuronidase histochemical assay and PC R amplification demonstrate that the overall trans-form ation rate of the explant reaches 29.4% ,and that,as compared with the strain of EHA105.the strain of LBA4404 is more suitable for the transform ation of Roselle explants because no acetosyringone is needed

Key words: Roselle, Agrobacterium tumCaciens, genetic transformation, β-glucuronidase, neomycin phosphotrans feraseⅡ