Extraction of Shark Cartilage Mucopolysaccharides by Enzym olysis and Their Composition Analysis

Journal of South China University of Technology (Natural Science Edition) ›› 2005, Vol. 33 ›› Issue (5): 74-77.

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Extraction of Shark Cartilage Mucopolysaccharides by Enzym olysis and Their Composition Analysis

Xiao Kai-jun Cai Miao-yan Guo Si-yuan Li Lin Wang Zhao-mei Luo Yuan-hong

Research Institute of Light Industry&Chemical Engineering,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China

Received:2004-06-21 Online:2005-05-25 Published:2005-05-25
Contact: 肖凯军(1969-)，男，副教授，主要从事活性多糖和分离工程方面的研究 E-mail:fekjxiao@seut.edu.cn
About author:肖凯军(1969-)，男，副教授，主要从事活性多糖和分离工程方面的研究
Supported by:
国家自然科学基金资助项目(20006005)；广州市科技攻关项目(2002Z3-E0321)

Abstract

Abstract:

The efects of the extraction times with one enzyme and the diferent treatments wit}l two enzymes on the extraction rate of mucopolysaccharides were investigated by carrying out orthogonal experiments.And the optimum hydrolysis conditions with trypsin and/or papain for the extraction of shark cartilage mucopolysaccharides were obrained.Moreover,the compositions of mucopolysaccharides extracted in diferent processes were analyzed by cellu-lose polyacetate strip and algarose gel electrophoresis.It can be seen from the experimental results that the degrada-tion rate and extraction rate for the extraction with tw o enzymes are higher than those for the extraction with one en-zyme.And the shark cartilage with papain·trypsin treatment can be hydrolyzed more efectively than that with tryp-sin·papain treatment,with a higher mucopolysaccharides extraction rate.Furthermore,the shark cartilag e mucopo-lysaccharides extracted with papain are purer than those extracted with trypsin.

Key words: papain, trypsin, shark cartilage, mucopolysaccharide, hydrolysis, extraction

Xiao Kai-jun Cai Miao-yan Guo Si-yuan Li Lin Wang Zhao-mei Luo Yuan-hong. Extraction of Shark Cartilage Mucopolysaccharides by Enzym olysis and Their Composition Analysis[J]. Journal of South China University of Technology (Natural Science Edition), 2005, 33(5): 74-77.

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Extraction of Shark Cartilage Mucopolysaccharides by Enzym olysis and Their Composition Analysis

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