Journal of South China University of Technology (Natural Science Edition) ›› 2005, Vol. 33 ›› Issue (12): 25-28.

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Production of Astaxanthin by Phaffia rhodozyma in Bioreactor

Zhu Ming-jun  Du Shun-tang  Wang Ju-fang  Liang Shi-zhong   

  1. College of Biological Science and Engineering,South China Univ.of Tech.,Guangzhou 510640,Guangdong,China
  • Received:2005-03-16 Online:2005-12-25 Published:2005-12-25
  • Contact: 朱明军(1969-)，男，博士，副教授，主要从事微生物发酵和生化工程研究 E-mail:mjzhu@scut.edu.cn
  • About author:朱明军(1969-)，男，博士，副教授，主要从事微生物发酵和生化工程研究
  • Supported by:

    广东省科技攻关项目(321-B28650)；广州市科技攻关项目(321-B2040150)

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Abstract:

High cell-density cultivation of Phaffia rhodozyma for the production of astaxanthin was carried out with an optimized medium in a 5-L stirred bioreactor in two stages.The early stage was mainly devoted to a rapid growth of the yeast cell,while in the later stage,when the cell content reached a certain level.urea was fed to promote the production of astaxanthin,thus improving the yield of astaxanthin.The following results are thus obtained:cell dry
weight,30.8 g/L,the yield of astaxanthin,28.1 mg/L(calculated as the mass of astaxanthin in unit.volume fer.mentation liquid),astaxanthin content,923.2 Ixg/g(calculated as the mass of astaxanthin in per gram of dry cell) and the yield of biomass to molasses,0.359.This research has laid a foundation for the scale-up production of astaxanthin by Ph~ffia rhodozyma.

Key words: Phaffia rhodozyma, astaxanthin, cultivation, bioreactor