Isolation, Purification and Structure Analysis of Sweet Potato Glycoprotein

Journal of South China University of Technology(Natural Science Edition) ›› 2004, Vol. 32 ›› Issue (9): 59-62.

Previous Articles Next Articles

Isolation, Purification and Structure Analysis of Sweet Potato Glycoprotein

Li Ya- na Lin Yong- cheng She Zhi- gang

Chemistry PostdoctorSun Yat -sen Univ．Guangzhou510275GuangdongChina

Received:2003-10-21 Online:2004-09-20 Published:2015-09-09
Contact: 李亚娜（1975－）女博士后主要从事天然产物方面的研究 E-mail:nayali＠sohu.com
About author:李亚娜（1975－）女博士后主要从事天然产物方面的研究

Abstract

Abstract: By using DEAE-52 cellulose and Sephadex G100 column chromatographysweet potato glycoprotein （SPG） was isolated and purified from sweet potatoand its structure was analyzed including the determination of the purity and molecular mass of SPG by SDS-PAGEthe identification of the linkage mode of glycopeptide bond by β-erase reactionthe analysis of monose composition by GLCand the deduction of the linkage type of glycosidic bond by IR spectra．The results indicate that SPG is a kind of conjugated protein with covalent bondtakes on white solid powder and is easily dissolved in waterwith a molecular mass of62ku and an O-linkage．It is also indicated that there areβ-glycosidic bonds and typical amido-carbonyl groups in SPG containing12．01％ protein （mass fraction）and that SPG saccharide chain is composed of fucosearabinosemannoseglucose and galactose．

Key words: sweet potato glycoprotein, isolation, purification, structure analysis

CLC Number:

Q282.7

Li Ya- na Lin Yong- cheng She Zhi- gang. Isolation, Purification and Structure Analysis of Sweet Potato Glycoprotein[J]. Journal of South China University of Technology(Natural Science Edition), 2004, 32(9): 59-62.

[1]	ZHENG Bisheng, WEI Ｒuijing, GUO Chaowan, et al. Study on Purification of Kapok Flavonoids by Macroporous Resin and Its Anti-acne Efficacy [J]. Journal of South China University of Technology(Natural Science Edition), 2022, 50(4): 119-128.
[2]	LING Yuhong, WU Shan, GU Jingxin, et al. A Comparative Study on Vibration Isolation Methods of Metro Superstructure#br# [J]. Journal of South China University of Technology(Natural Science Edition), 2021, 49(4): 1-8.
[3]	MENG Hailin LIU Furong HE Jingyu ZHANG Wenyan ZHANG Honggang CUI Jinming. Isolation and Identification of Heterotrophic Nitrification Bacterium and Nitrification Performance Analysis of Mixed Culture of Multi-strains [J]. Journal of South China University of Technology (Natural Science Edition), 2019, 47(9): 113-120.
[4]	YANG Gang DU Linglong LIANG Laiyu CHEN Kai. Design and Experimental Ｒesearch of Oil Bladder Type Pressure Simulation Control System [J]. Journal of South China University of Technology (Natural Science Edition), 2018, 46(2): 8-13,21.
[5]	SHANGGUAN Wen-bin CHEN Cheng-feng LI Li-ping YU Hong QI Zhen-ning. Analysis of Vibration Isolation Performances of Vehicle Suspension System Based on Vibrational Power Flow Method [J]. Journal of South China University of Technology (Natural Science Edition), 2017, 45(2): 23-29,38.
[6]	Zha Xiao-xiong Zuo Yang Liu Le Wang Xin-jie. Analysis of Mechanical Properties of Container Structure Under Earthquake Action [J]. Journal of South China University of Technology (Natural Science Edition), 2015, 43(7): 92-99.
[7]	Ren Jiao-yan Lai Ting Jiang Yan-qing Lu Yun-jun Liu Peng Liao Wen-zhen. Enzymatic Preparation，Separation，Purification and Antioxidant Activity Evaluation of Whey Protein Peptides [J]. Journal of South China University of Technology (Natural Science Edition), 2015, 43(2): 1-7.
[8]	Zhao Qiang-zhong Feng Meng-ying Lin Lian-zhu Zhao Mou-ming. Separation，Identification and Antioxidant Activity of Glycoproteins from Millettia Speciosa Champ． [J]. Journal of South China University of Technology (Natural Science Edition), 2015, 43(11): 8-15.
[9]	Fan Xiao- jiang Lei Ying Zhang Xi- hui Chao Meng Hiroshi Noguchi. Investigation into a New Integrated Water Purification Process of Half Coagulation/Ozonation/Ceramic Membrane Filtration [J]. Journal of South China University of Technology (Natural Science Edition), 2014, 42(5): 54-59.
[10]	Xu Qiao-ning Yu Feng Zhou Hua Yang Hua-yong . Sensor Fault Detection and Isolation for an Electro- Hydraulic Servo System Based on Robust Observer [J]. Journal of South China University of Technology (Natural Science Edition), 2014, 42(11): 31-39.
[11]	Li Li-feng Ren Li Liu Sa Wang Ying-jun. Production and Cell Compatibility of Medical Bacterial Cellulose [J]. Journal of South China University of Technology (Natural Science Edition), 2014, 42(10): 44-49,56.
[12]	Lou Meng-lin Jia Bao-yin Zong Gang Shan Tao-tao. Measurement and Analysis of Subway-Induced Vibration of Buildingwith Vibration Isolation Using Continuous Concrete Wall [J]. Journal of South China University of Technology (Natural Science Edition), 2013, 41(3): 50-62.
[13]	Meng He-cheng Bi Shui-lian Yan He Shi Lei. Isolation of Campylobacter Strains in Poultry Products and Genotyping Identification of Strains by Means of PFGE and DGGE [J]. Journal of South China University of Technology(Natural Science Edition), 2012, 40(5): 149-154.
[14]	Liu Xiao-lan Zhang Wen-shu Zheng Xi-qun Shen Yuan Sun Ying. Isolation and Purification of Novel Fibrinolytic Enzymes Obtained from Fermentation of Cordyceps militaris [J]. Journal of South China University of Technology(Natural Science Edition), 2012, 40(5): 107-114.
[15]	Fu Kai Fu Shi-yu Zhang Li Zhou Pan-deng Zhan Huai-yu. Production and Enzymatic Properties of Laccase from a Subtropical Wood-Rot Fungus [J]. Journal of South China University of Technology (Natural Science Edition), 2011, 39(9): 152-157,164.

Isolation, Purification and Structure Analysis of Sweet Potato Glycoprotein

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments