Journal of South China University of Technology(Natural Science Edition) ›› 2004, Vol. 32 ›› Issue (5): 42-46.

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Cellular Immunity Induced by gD2 DNA Vaccine

Han Shuang-yan1  Guo Yong1  Yang Hui-lan2  Wang Jie3 Yang Tai-cheng3   

  1. 1．College of Food and Biological Engineering‚South China Univ．of Tech．‚Guangzhou510640‚Guangdong‚China；
    2．Dept．of Dermatology‚Guangzhou General Hospital of Guangzhou Military Command‚Guangzhou510010‚Guangdong‚China；
    3．Dept．of Medical Research‚Guangzhou General Hospital of Guangzhou Military Command‚
    Guangzhou510010‚Guangdong‚China
  • Received:2003-09-23 Online:2004-05-20 Published:2015-09-08
  • Contact: Han Shuang-yan （born in1976）‚female‚Ph．D．candidate‚mainly researches on herpes simplex virus Ⅱ DNA vaccine． E-mail:hanshuangyan＠21cn.com
  • About author: Han Shuang-yan （born in1976）‚female‚Ph．D．candidate‚mainly researches on herpes simplex virus Ⅱ DNA vaccine．
  • Supported by:
    Supported by Guangdong Provincial Natural Science Foundation （010795）

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Abstract:  pcDNA3-gD‚the constructed Genital Herpes gD DNA vaccine （gD2DNA vaccine）‚was transfected into mammalian cell COS-7‚and the expression of gD gene was investigated．BALB／c mice were then immunized by pcDNA3-gD．The proliferation of spleen cell‚the activity of cytotoxic T cell （CTL） and CD4＋ and CD8 ＋populations were respectively analyzed．The results show that pcDNA3-gD plasmid can express the gene of gD in cell COS-7‚that the spleen cell of mice immunized by pcDNA3-gD plasmid is of an increased proliferation activity‚that the activity of CTL in the experiment group （immunized by pcDNA3-gD plasmid） is37．69％which is obviously higher than that of the control group （immunized by pcDNA3plasmid） and the naive group （immunized by normal saline）‚and that the percentage of CD4＋cells in the experiment group increases to33．38％ which is greater than that of the control group and the naive group．

Key words: immunity, gD2DNA vaccine, cytotoxic T cell, CD4＋, CD8＋, herpes

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